人类精子KSper通过细胞内pH化和CatSper介导的Ca2+信号传递而生理上被激活
Hang Kang1, Huafeng Wang2, Jie Wu1
1Institute of Reproductive Medicine, Medical School, Nantong University, Nantong, Jiangsu, China.
The Journal of biological chemistry
|September 24, 2025
概括
生理学pH值变化和水平激活人类精子通道 (hKSper),对精子功能至关重要. 这项研究阐明了细胞内pH和对hKSper的调节,提高了对精子受精能力的理解.
科学领域:
- 生殖生物学 生殖生物学
- 离子通道生理学 离子通道生理学
- 精子功能 精子功能
背景情况:
- 精子膜潜力 (Vm) 通过精子特定的K+通道 (KSper) 的超极化对于受精能力至关重要.
- 人类KSper (hKSper) 通过生理细胞内pH和Ca2+ ([Ca2+]i) 的精确调节仍然不清楚,这是由于hKSper的低pH敏感性和Ca2+亲和力.
研究的目的:
- 通过生理刺激来研究人类原生精子KSper (hKSper) 的激活.
- 阐明细胞内pH和Ca2+在调节hKSper活动和精子Vm中的作用.
主要方法:
- 对人类精子进行了定量Vm度和电流记录.
- 实验涉及操纵细胞外pH值和使用孕激素作为CatSper激动剂.
- 用药学抑制剂和Ca2+化剂来剖析通道功能.
主要成果:
- 细胞外pH化激活了hKSper,导致显著的Vm超极化 (∼20mV).
- 孕激素诱导的剂量依赖的Vm超极化,依赖于hKSper和CatSper.
- hKSper活动增强了[Ca2+]i水平约100nM,表明对静止Ca2+的敏感性.
结论:
- 细胞内pH和Ca2+升高在生理上激活人类精子中的hKSper.
- 这些发现澄清了hKSper的调控机制,特别是它与Ca2+信号的相互作用.
- 了解hKSper调节可以了解精子受精能力和生育治疗的潜在目标.
关键词:
在Ca2+) 信号传输中.在CatSper上使用.hKSper 在这里.人类精子特定的K(+) 通道.膜潜力是一个潜在的潜力.的化 pH 的化.休息[Ca2+) ](i) 的水平.精子特定的Ca2+) 通道.更多相关视频
05:44Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm
Published on: March 1, 2019
8.7K
19:26Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging
Published on: May 24, 2013
20.7K
相关概念视频
Spermatogenesis
121.9K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
121.9K
Spermatogenesis
8.9K
Spermatogenesis is a complex process that involves the development of sperm cells from undifferentiated stem cells in the seminiferous tubules of the testes. The process is essential for the production of mature and functional sperm cells that are capable of fertilizing an egg.
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...
8.9K
Sperm Transport
3.1K
The journey of sperm from its origin to the point of ejaculation begins within the seminiferous tubules of the testis. Here, Sertoli cells produce fluid that propels non-motile sperm through a series of conduits, starting with the straight tubules leading to the rete testis. This interconnected network of tubules acts as the initial pathway for sperm, guiding them into the efferent ductules and then into the epididymis for maturation.
The maturation phase occurs in the epididymis, where sperm...
The maturation phase occurs in the epididymis, where sperm...
3.1K
Fertilization
89.8K
During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...
89.8K
Sperm Structure and Semen Composition
10.4K
During ejaculation, males release around 2-5 milliliters of semen, which is a complex mixture of mature sperm and various fluids produced by accessory glands. The mature sperm cells measure approximately 60 micrometers in length and consist of a head, neck, midpiece, and tail. The head is flattened and tapered, measuring about 4 to 5 micrometers in length. It contains a nucleus with condensed chromosomes and an acrosome, a cap-like structure filled with enzymes essential for penetrating the...
10.4K
Feedback Regulation of Calcium Concentration
3.9K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
3.9K
