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欧洲试点实验室间对Mopox病毒全基因组测序的比较研究

Jonas Fuchs¹, Claire Bertelli², Trestan Pillonel²

¹Institute of Virology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases

|September 24, 2025

概括

马普克斯病毒 (MPXV) 的全基因组测序 (WGS) 显示了2022年疫情株的高精度. 然而,其他MPXV类的结果的变化凸显了基因组监测中标准化质量评估的必要性.

科学领域:

病毒学病毒学
基因组流行病学基因组流行病学
公共卫生微生物学

背景情况:

自2022年以来,在全球范围内出现了不同的Mpox病毒 (MPXV) 菌群,使流行病学跟踪复杂化.
全基因组测序 (WGS) 对于患者管理和监测MPXV传播至关重要.
一项实验室间比较研究旨在评估MPXV WGS的性能.

研究的目的:

评估不同实验室在Mopox病毒全基因组测序方面的表现.
确定MPXV基因组监测的潜在挑战和改进领域.
评估各种MPXV菌株类和子类鉴定的准确性.

主要方法:

针对MPXV类I和II的非传染性DNA样本,以及牛病毒 (CPXV) 的对照样本,分发给了八个欧洲实验室.
参与实验室使用他们喜欢的WGS管道,模仿真实世界的条件.
报告的数据包括测序管道,基因组覆盖范围和MPXV/CPXV识别.

主要成果:

七个实验室提交了结果,在很大程度上准确地识别了MPXV菌群.
对于MPXV类IIb 2022菌株,报告了具有最小变异的高质量基因组.
在非clade IIb MPXV菌株的基因组重建中观察到显著的变异性;CPXV被三个实验室正确识别.

结论:

虽然MPXV类IIb 2022菌株序列可靠,但MPXV类I和IIa的挑战仍然存在.
针对性测序和生物信息学分析对某些MPXV类型构成潜在的陷.
标准化的外部质量评估研究对于强大的MPXV基因组监测至关重要.

关键词:

实验室间比较研究的研究.蒙博克斯病毒 (Mopox virus) 是一种病毒.麻疹病毒 (poxvirus) 是一种病毒.准备的情况准备的情况整个基因组的测序.

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