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Updated: Jun 20, 2026

A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis
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亲和性净化质谱测试,以确定N端乙转移酶NAA40的核蛋白相互作用

Ariel Klavaris1, Ann-Christine König2, Constantinos Demetriades1

  • 1Epigenetics and Gene Regulation Laboratory, Department of Biological Sciences, University of Cyprus, Nicosia, Cyprus.

Methods in enzymology
|September 24, 2025
PubMed
概括

N-终端乙转移酶40 (NAA40) 是一种修改基因组的酶. 这项研究使用亲和性净化与质谱学 (AP-MS) 相结合,确定了NAA40的新核蛋白相互作用,以更好地了解其核功能.

关键词:
亲和力净化 - 质谱学质谱学免疫沉是一种免疫沉.在N端的乙化.NAA40 NAA40 的时间.NATs 是一个很好的解决方案.

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相关概念视频

Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...