相关实验视频
Updated: Jan 17, 2026

08:30
MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR
Published on: May 16, 2012
25.0K
snoRNP RRP9 通过SQSTM1促进前列腺癌细胞的增殖和迁移
Butang Li1,2, Lihui Shen1, Hui Huang2
1Department of Urology, Ningbo Hangzhou Bay Hospital, Zhejiang, 315336, China.
Advanced biology
|September 25, 2025
概括
小核核核核糖核蛋白U3 snoRNA相互作用蛋白2 (RRP9) 促进前列腺癌的生长. 针对RRP9-Sequestome-1 (SQSTM1) 途径可能提供新的前列腺癌疗法.
科学领域:
- 分子生物学分子生物学
- 癌症研究 癌症研究
- 生物化学 生物化学
背景情况:
- 小核RNAs (snoRNAs) 与癌症的不良结果有关.
- 在前列腺癌 (PCa) 中,snoRNAs和小核核核糖核蛋白复合体 (snoRNP) 的作用尚不清楚.
- RRP9是一种与U3snoRNA相互作用的蛋白质,正在研究其在PCa.中的作用.
研究的目的:
- 为了研究RRP9在前列腺癌发病过程中的作用.
- 确定RRP9的结合伙伴,并了解其在PCa细胞中的作用机制.
主要方法:
- 在PCa细胞系 (DU-145,PC-3) 中使用shRNA击败了RRP9.
- 评估了细胞增殖,迁移和体内瘤生长.
- 使用拉下,MALDI-TOF/TOF和共免疫沉试验确定了蛋白质与蛋白质的相互作用.
主要成果:
- 在体外和体内抑制RRP9敲击PCa的生长和迁移.
- 发现RRP9与Sequestome-1 (SQSTM1,p62) 发生相互作用.
- 过度表达SQSTM1挽救了RRP9敲击的抑制作用,表明它的关键作用.
结论:
- 在前列腺癌中发现了RRP9-SQSTM1轴在前列腺癌中的新瘤作用.
- 通过与SQSTM1.1的相互作用,RRP9促进PCa细胞的增殖和迁移.
- 针对RRP9-SQSTM1轴为PCa.提供了一个潜在的治疗策略.
相关概念视频
Abnormal Proliferation
5.1K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.1K
mTOR Signaling and Cancer Progression
4.6K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
4.6K
The Ras Gene
7.0K
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
Ras is a...
7.0K
Small GTPases - Ras and Rho
5.2K
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
Three regulatory proteins control their activity:
5.2K
Rous Sarcoma Virus (RSV) and Cancer
6.2K
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
6.2K

