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[用于生产L-氨酸的Corynebacterium glutamicum的代谢工程]

Hailang Yu¹, Jia Liu¹, Xiaomin Li¹

¹Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.

Sheng wu gong cheng xue bao = Chinese journal of biotechnology

|September 25, 2025

概括

这项研究对Corynebacterium glutamicum进行了改造,以提高L-氨酸的产量. 系统代谢工程为工业应用实现了这种必需氨基酸的高位数和产量.

科学领域:

生物技术是生物技术.
代谢工程是代谢工程.
微生物生产微生物生产

背景情况:

L-氨酸是一种必不可少的分支链氨基酸,对料,制药和食品行业至关重要.
优化L-瓦林的生产对于满足工业需求至关重要.
谷氨酸菌 (Corynebacterium glutamicum) 是一种成熟的微生物宿主,用于氨基酸生产.

研究的目的:

为了提高Corynebacterium glutamicum中的L-氨酸生产性能.
通过系统的代谢工程来提高标位,产量和生产力.
为了实现高效的工业规模L-氨酸生物合成.

主要方法:

用Corynebacterium glutamicum的代谢工程来增强前体的供应,合成和运输.
菌株发展 (VH-9和VH-18) 专注于L-氨酸生物合成途径.
优化发酵条件以最大限度地提高L-氨酸的产量.

主要成果:

工程菌株VH-9实现了L-氨酸标位,产量和76.6g/L,0.45g/g和2.39g/L·h的生产率.
进一步改造的VH-18菌株通过增强葡萄糖吸收和能源供应,改善了L-瓦林的产生 (82.7 g/L,0.52 g/g,2.58 g/L·h).
对VH-18的优化发酵条件导致最终L-氨酸标位为88.7g/L,产量为0.54g/g,生产率为2.77g/L·h.

结论:

系统代谢工程显著增强了Corynebacterium glutamicum中的L-氨酸的产生.
开发的菌株和优化过程证明了高效的L-氨酸生物合成.
这一战略为工业L-氨酸制造提供了一个强大的平台.

关键词:

在Corynebacterium glutamicum的研究中,我们发现这是一种L-氨酸.发酵优化优化发酵的优化代谢工程是代谢工程.

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