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Updated: Jan 6, 2026

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
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High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

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高面积和体积吞吐量并行二光子光显微镜使用光倍增器阵列

Vincent D Ching-Roa1, Chi Z Huang1, Michael G Giacomelli1

  • 1Department of Biomedical Engineering, University of Rochester, Rochester, New York 14627, USA.

Photonics research
|September 25, 2025
PubMed
概括

这项研究引入了一种更快的双光子光显微镜 (TPFM) 方法,通过将并行扫描与定制探测器相结合. 这项创新显著提高了大型生物样本的成像速度,克服了以前的局限性.

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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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