相关实验视频

Updated: Jan 17, 2026

Murine Dermal Fibroblast Isolation by FACS

Murine Dermal Fibroblast Isolation by FACS

Published on: January 7, 2016

糖尿病伤口愈合中的免疫微环境和纤维细胞亚群

Jing Gan^1,2, Yuanrong Chen¹, Lu Tang³

¹Department of Endocrinology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325035, China.

|September 25, 2025

概括

糖尿病足 (DFU) 显示出独特的细胞变化,影响伤口愈合. 了解这些免疫和纤维细胞细胞的改变为DFU治疗提供了新的治疗点.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
伤口治愈研究研究伤口治愈研究

背景情况:

糖尿病足 (DFU) 是一个重大的临床挑战,因为伤口愈合受损.
在DFU愈合缺陷背后的细胞机制需要进一步阐明.

研究的目的:

在伤口修复过程中调查DFU微环境的细胞景观.
确定特定的免疫和纤维细胞变化,有助于DFU中受损的愈合.

主要方法:

来自DFU患者的颗粒组织的单细胞RNA测序 (scRNA-seq).
对免疫细胞种群的分析,包括单细胞和T细胞.
评估纤维细胞亚种群及其基因表达特征.
光成像用于验证蛋白质水平上的转录发现.

主要成果:

识别具有特定亚群的炎症单细胞轴 (CD14+CCL2+和FCN1+CCR2+).
在T细胞群体中表现出不平衡,调控性T细胞 (IL2RA+FOXP3+) 和扩展效应性T细胞 (CD8+GZMK+) 的减少.
观察了参与细胞外基质生产的特定纤维细胞亚群的减少 (COL6A1+COL6A3+和COL7A1+COL10A1+).
通过光成像,通过蛋白质水平确认scRNA-seq发现.

结论:

该DFU修复微环境在免疫和纤维细胞中表现出明显的细胞变化.
确定了特定的免疫纤维细胞相互作用,可能是DFU中观察到的受损愈合的基础.
这些发现为开发针对性治疗策略的DFU治疗提供了基础.

关键词:

免疫学免疫学免疫学微观环境是一个微观环境.文字转录学 (Transcriptomics) 是一个学科.

更多相关视频

Protocol to Create Chronic Wounds in Diabetic Mice

Protocol to Create Chronic Wounds in Diabetic Mice

Published on: September 25, 2019

Prospective, Randomized, and Controlled Study of a Human Umbilical Cord Mesenchymal Stem Cell Injection for Treating Diabetic Foot Ulcers

Prospective, Randomized, and Controlled Study of a Human Umbilical Cord Mesenchymal Stem Cell Injection for Treating Diabetic Foot Ulcers

Published on: March 3, 2023

相关概念视频

Introduction to Fibroblasts

Introduction to Fibroblasts

Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...

The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

Phases of Wound Repair

Phases of Wound Repair

Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...