相关实验视频
Updated: Jan 17, 2026
12:59
Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
Published on: September 26, 2013
35.5K
在2型糖尿病的活性结核病中,循环Th17细胞增加和CD4T细胞成熟和分化发生变化:试点研究
Paul Ogongo1, Yoscelina E Martinez-Lopez2, Anthony Tran1
1Division of Experimental Medicine, University of California, San Francisco, San Francisco, CA, United States.
Frontiers in immunology
|September 25, 2025
概括
2型糖尿病 (T2D) 通过减少原始细胞和增加促炎子集,显著改变CD4 T细胞对结核病 (TB) 的反应. 结核病-T2D患者的这些免疫变化可能会使结核病的结果恶化.
科学领域:
- 免疫学 免疫学 免疫学
- 传染性疾病 传染性疾病
- 内分泌学 在内分泌学.
背景情况:
- 2型糖尿病 (T2D) 是结核病 (TB) 的重要危险因素.
- 由于研究方法不一致,在T2D相关的结核病中了解T细胞功能障碍具有挑战性.
- 单独的细胞因子概况可能无法完全捕捉TB患者的T细胞复杂性.
研究的目的:
- 为了确定表型和ex vivo CD4 T 细胞对 Mycobacterium 结核病 (Mtb) 抗原的反应变化,在结核病患者中,有或没有T2D.
- 利用基于细胞表面标记的细胞因子无关的方法来评估T细胞分化.
- 研究T2D对T细胞成熟,分化和Mtb抗原响应性的影响.
主要方法:
- 试点研究评估来自有或没有T2D的结核病患者的外周血液单核细胞 (PBMC).
- 高参数光谱流细胞计用于详细的T细胞分析.
- 使用经过验证的细胞表面标记物对T细胞分化的细胞因子不可知评估.
主要成果:
- T2D状态显著改变了CD4 T细胞特性,独立于CD4/CD8 T细胞数量.
- 与只有结核病的患者相比,结核病-T2D患者表现出较少的天真CD4T细胞.
- 在TB-T2D患者中观察到CD4 T细胞对Mtb抗原的反应增加,Th1升高,以及三种Th17细胞子集.
结论:
- T2D诱导CD4 T细胞的改变,这可能会对结核病的结果产生负面影响.
- 一个由减少的天真和增加激活的CD4 T细胞特征的促炎环境表明结核病控制受损.
- 针对这些免疫功能障碍,可以为结核病预防提供宿主导疗法,并改善T2D患者的治疗结果.
相关概念视频
01:29Pulmonary Tuberculosis I
838
Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
838
01:28Pulmonary Tuberculosis II
1.4K
Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
1.4K
01:24T Cell Types and Functions
2.2K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
2.2K
01:26Pulmonary Tuberculosis IV
478
Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
478
01:31Pulmonary Tuberculosis III
937
Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories:
The first classification is based on the development of the disease, and it includes the following categories:
937
01:28Pulmonary Tuberculosis V
540
Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the...
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the...
540