在大型黄色鱼 (Larimichthys crocea) 中嗅觉检测ursodeoxycholic acid (UDCA):反应,通路和受体功能
Aijun Zhu1,2, Xiaolin Zhang1, Xiaojun Yan1,2
1National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan 316022, People's Republic of China.
The Journal of experimental biology
|September 25, 2025
概括
Ursodeoxycholic 酸 (UDCA) 强烈吸引大型黄色鱼,作为一种强大的嗅觉暗示. 这一发现增强了对鱼类化学感应的理解,并有助于保护工作.
科学领域:
- 海洋生物学 海洋生物学
- 化学生态化学生态学
- 鱼类生理学 鱼类生理学
背景情况:
- 嗅觉沟通对于鱼类来说至关重要,尤其是在视力有限的情况下.
- 胆酸在水生环境中既可以作为消化剂,也可以作为关键的嗅觉信号.
研究的目的:
- 研究ursodeoxycholic acid (UDCA) 作为临灭绝的大黄鱼 (Larimichthys crocea) 的嗅觉吸引剂.
- 阐明涉及UDCA检测和行为反应的分子机制和信号通路.
主要方法:
- 电生理学记录以确定嗅觉反应值.
- 行为测试将UDCA的吸引力与其他胆酸进行比较.
- 转录组分析和药理疗法,以确定受体参与和信号通路.
- 功能性受体表达测试用于验证信号机制 (与cAMP).
主要成果:
- UDCA引起了强烈的,依赖于度的嗅觉反应,检测值低 (10^-12 M).
- 与结构相似的胆汁酸相比,UDCA的吸引力显著更高.
- 观察到分阶段的受体参与:V2R1上调和PLC通路激活先通过cAMP信号诱导OR11A1,TAAR13C-10和OR52L2.
- V2R1调解信号,而OR11A1,TAAR13C-10和OR52L2利用cAMP信号进行UDCA检测.
结论:
- UDCA是大型黄色鱼的强烈嗅觉吸引剂,具有特定的受体和信号通路调解其检测.
- 这项研究提供了对鱼类UDCA化学感应的全面了解.
- 这些发现对危鱼类的保护和可持续的渔业管理有影响.
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