一个Campylobacter jejuni传感器酸酶驱动的两组系统集成了代谢性酸作为信号传导中的线索
Nestor Ruiz1, David R Hendrixson1
1Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9048.
概括
坎比洛巴克特 (Campylobacter jejuni) 的BumSR系统使用乙酸和碳酸作为BumR响应调节器的phosphodonors,整合宿主殖民的代谢线索.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 细菌的二元信号传导系统 (TCS) 通过传感器激酶和响应调节器调节细胞反应.
- 坎皮洛巴克特 (Campylobacter jejuni) 的BumSR TCS是独一无二的,其传感器 (BumS) 仅作为酸酶,需要外部酸为其反应调节器 (BumR).
研究的目的:
- 在Campylobacter jejuni.中识别BumR的自然体内活体色素体.
- 阐明BumSR TCS如何整合各种环境和代谢信号以有效的宿主殖民.
主要方法:
- 基因查和选择以识别光鱼.
- 生理学测试用于评估系统功能.
- 生物化学分析证实了色素的活性.
主要成果:
- 乙酸 (AcP) 和碳酸 (CP) 被确定为BumR的主要体内酸.
- AcP和CP来自氨基酸代谢,这表明营养物质的可用性和信号传导之间存在联系.
- BumSR系统集成了宿主衍生代谢物线索 (影响BumS酸酶活性) 和营养衍生线索 (AcP/CP,影响BumR酸化).
结论:
- BumSR TCS 集成了来自宿主环境和内部代谢状态的信号.
- 这种多cue集成允许Campylobacter jejuni根据营养的可用性和宿主肠道内的空间位置优化其殖民策略.
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