在CRP/FNR家族的DNA结合域中的功能演变
Madhumitha Krishnaswamy1,2, Aswin Sai Narain Seshasayee1
1National Centre for Biological Sciences, Tata Institute of Fundamental Research, GKVK Campus, Bengaluru, India.
细菌转录因子 (TF),如循环AMP受体蛋白 (CRP) 和酸盐和酸盐还原酶 (FNR) 调节器,在早期进化基基DNA结合. 转录激活是后来进化的,并且仅限于特定的细菌群体.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 进化生物学 进化生物学
背景情况:
- 转录因子 (TFs) 对于细菌适应环境变化至关重要.
- 循环AMP受体蛋白 (CRP) 和酸盐和酸盐还原酶 (FNR) 调节器家族是TF进化的一个例子.
- 了解TF的功能多样化是了解细菌调节网络的关键.
研究的目的:
- 调查CRP/FNR TF家族在 prokaryotes 的进化多样化和功能收益.
- 确定这个家族内的DNA结合和转录激活功能的祖先状态和进化轨迹.
主要方法:
- 在6000个 prokaryotic 基因组中对CRP/FNR DNA 结合域进行基因组学分析.
- 对现存和在基重建的祖先序列的残留保护分析.
- 对TF-RNA聚合酶相互作用接口的比较分析.
主要成果:
- 祖先的CRP/FNR TF是一种多样化的"CRP类"蛋白质,与现代的CRP或FNR类不同.
- 基因特定的DNA结合残留物在整个家族的历史中被保存.
- 通过激活区域1 (AR1) 和其RNA聚合酶相互作用部位的转录激活仅限于Gammaproteobacteria,与大肠杆菌CRP共同进化.
结论:
- 基因特异性DNA结合是CRP/FNR家族的一个古老特征.
- 在大肠杆菌CRP中看到的I类转录激活是一种较新的,在遗传学上有限的创新.
- TF的功能进化涉及到古老的保存机制和特定的创新.
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