可转移元素介导的Cis调节推动了dmrt1作为黑鱼中候选主性决定基因的进化
Jiamin Guo1, Gen Hua Yue2, Jiale Li1,3
1Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, No. 999 Hucheng Huan Road, Lingang New City, Shanghai 201306, China.
Molecular biology and evolution
|September 25, 2025
概括
黑鱼中的可转移元素促进了dmrt1作为男性决定基因的进化. 在dmrt1促进体中的这些元素驱动Y特定基因激活,揭示了性别决定进化的新机制.
科学领域:
- 进化生物学是进化的生物学.
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
背景情况:
- 脊椎动物的性别决定显示了具有多种主性性别决定基因 (MSD) 的进化可塑性.
- dmrt1是一种剂量敏感基因,通过基因重复或等位基因多样化反复被招募为MSD基因.
- 进化转变在性别决定中的生物化学基础,特别是通过等位基多样化,在很大程度上是未知的.
研究的目的:
- 调查dmrt1作为黑鱼中男性决定基因的进化背后的分子机制.
- 确定负责Y特定dmrt1激活的遗传元素.
- 了解移动遗传元素在性别确定系统起源中的作用.
主要方法:
- 为 XX 和 XY 黑鱼生成了基于单元型解析的基因组组件.
- 在染色体4上确定了一个含有dmrt1的候选性决定位点.
- 进行了功能性测试,以分析dmrt1促进体中Y特异插入的调节作用.
主要成果:
- 染色体4上含有dmrt1的~40kb区域被确定为候选的性别决定位置.
- 在dmrt1促进体中发现了两个Y特异插入:一个13.4kb的重复元素和一个11-bp的动机.
- 这些插入驱动dmrt1的早期,基因特异性上调,在13.4kb插入中,可转移元素作为Y特异性激活的cis调节模块.
结论:
- 可转移元素调解了一项监管创新,通过等位基因多样化促进dmrt1作为男性决定基因的进化.
- 这项研究提供了关于移动遗传元素如何推动脊椎动物性别决定系统的起源和多样化的见解.
- 这些发现突出了Y特定基因调节的新机制,有助于性别决定的进化.
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