通过弱基同步膜分离获得的大豆蛋白分离物对纳米乳液稳定性的影响:与性提取/异电沉和盐提取的比较
Jiaqi Shao1, Jing Yang2, Ping Wu1
1Key Laboratory of Oilseeds Processing, Ministry of Agriculture, Hubei Research Center of Oil and Plant Protein Engineering Technology, Oil Crops and Lipids Process Technology National & Local Joint Engineering Laboratory, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, Hubei 430062, PR China.
Food chemistry
|September 25, 2025
概括
与 (AE) 或盐 (SE) 方法相比,膜分离 (MS) 提取产生更高的大豆蛋白分离物 (SPI) 恢复率,并产生更稳定的纳米乳液. 在食品应用中,MS-SPI具有卓越的乳化特性.
科学领域:
- 食品科学与技术 食品科学与技术
- 蛋白质化学 蛋白质化学
- 材料科学 材料科学 材料科学
背景情况:
- 豆蛋白分离物 (SPI) 对于稳定的食品乳液配方至关重要.
- 需要有效的提取方法来最大限度地提高SPI产量和功能.
- 像提取 (AE) 和盐提取 (SE) 这样的现有方法在产量和蛋白质质量方面存在局限性.
研究的目的:
- 为了比较三种大豆蛋白分离物 (SPI) 提取方法的效率:膜分离 (MS),提取 (AE) 和盐提取 (SE).
- 评估通过不同的方法提取的SPI对纳米乳液物理化学稳定性的影响.
- 为了确定MS-SPI作为纳米乳液基础食品的乳化剂的适用性.
主要方法:
- 来自MS,AE和SE提取技术的蛋白质产量的比较.
- 用大豆蛋白分离物 (SPI) 稳定纳米乳液的制备和表征.
- 在加速储存条件下 (15天55°C) 评估纳米乳液稳定性 (颗粒大小,Turbiscan稳定性指数).
- 使用冷扫描电子显微镜 (cryo-SEM) 和传输电子显微镜 (TEM) 的微结构分析.
主要成果:
- 与AE (38%) 和SE (27%) 相比,膜分离 (MS) 提取实现了显著更高的蛋白质回收 (53%).
- 稳定MS-SPI的纳米乳液表现出优越的物理化学稳定性,具有较小的颗粒大小和更高的稳定性指数,与商业酸可比.
- 化SEM/TEM显示,MS-SPI在油水界面形成了一个更紧密,更均的界面膜,增强了乳液稳定性.
结论:
- 弱基同步膜分离 (MS) 是一种高效的方法,用于提取具有卓越乳化能力的大豆蛋白分离物 (SPI).
- 作为一种稳定的乳化剂,MS-SPI在开发先进的以纳米乳液为基础的食品产品方面表现出极好的潜力.
- MS-SPI稳定纳米乳液的增强稳定性归因于强大的界面膜的形成.
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