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一种醇激活的近红外光H2S供体,用于可视化H2S释放及其应用

Jicheng Wang¹, Xuan Wang¹, Nianwei Wang¹

¹Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, and School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, PR China.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

|September 25, 2025

概括

研究人员开发了AD-SH,一种新的近红外光硫化 (H₂S) 供体. 这种H₂S捐赠体对多种细胞内生物醇有反应,使H₂S释放的实时跟踪和可视化成为可能.

科学领域:

生物化学生物化学
化学生物学化学生物学
医疗成像医学成像

背景情况:

硫化 (H2S) 是一种具有不同生理作用的关键内源信号分子.
H2S 捐赠者被研究为治疗剂,但现有的有机捐赠者通常仅依赖氨酸,冒着细胞醇失衡的风险.
开发对多种生物乙醇有反应的H2S捐赠体对于安全有效的治疗应用至关重要.

研究的目的:

开发一种新的近红外 (NIR) 光硫化 (H₂S) 供体.
创建一个对多个细胞内生物醇有反应的H2S供体,克服了依赖氨酸的系统的局限性.
为了实现实时跟踪和可视化生物系统中的H2S释放.

主要方法:

合成AD-SH,一种基于二烯盐的NIR光H2S供体.
评估AD-SH选择性和对各种细胞内生物醇 (氨酸,谷氨酸,同类氨酸) 的敏感性.
对活细胞成像和小鼠体内成像进行AD-SH的评估,包括生物相容性和光稳定性研究.

主要成果:

AD-SH对多种细胞内生物醇具有很高的选择性和灵敏性,而对氨酸没有偏好的耗尽.
AD-SH的NIR光允许实时跟踪和可视化H₂S释放.
在小鼠中,AD-SH表现出极好的生物相容性,光稳定性和体内成像性能.

结论:

AD-SH是一种新的,多功能的NIR光H2S供体,对多种生物醇有反应.
AD-SH为活细胞成像和动态H₂S释放跟踪提供了一个有前途的工具.
AD-SH的发展解决了以前的H2S捐赠者的局限性,并显示了治疗和诊断应用的潜力.

关键词:

生物成像是一种生物成像.光是一种光效应.H(2) S 是一个捐赠者.醇 (Thiol) 是一种

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