探索潜在的枢纽基因和分子机制,将心脏癌与Sjögren综合征联系起来,基于全面的生物信息学分析和机器学习
Meng Qian1, Ying Chen1, Zhenxiang Wang1
1Department of Gastroenterology, Tongji Institute of Digestive Disease, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
The journal of gene medicine
|September 25, 2025
概括
这项研究确定了三个关键基因 (E2F3,CHIA,SCNN1B) 和Sjögren综合征 (SS) 患者心脏癌 (CC) 的预测模型. 这些发现突出了两种疾病共同的潜在免疫相关机制.
科学领域:
- 在瘤学瘤学.
- 遗传学 是一个遗传学.
- 免疫学 免疫学 免疫学
背景情况:
- 心脏癌 (CC) 是一个日益严重的全球健康问题.
- 胃食道逆流性疾病是已知的CC风险因素.
- 斯约格伦综合征 (SS) 患者经常表现出食道运动问题.
研究的目的:
- 确定CC和SS中常见的枢纽基因和分子通路.
- 在SS患者中开发CC风险的预测模型.
主要方法:
- 使用基因表达总 (GEO) 数据集进行分析.
- 进行了差异基因表达 (DEGs) 和加权基因共表达网络分析 (WGCNA).
- 应用机器学习算法 (RF,LASSO,SVM-RFE,XGBoost) 来识别枢纽基因.
- 构建并验证了用于CC风险预测的nomogram.
主要成果:
- 确定了60个共同的基因,这些基因丰富于细胞周期,异生物反应和p53信号通路.
- 选了三个枢纽基因 (E2F3,CHIA,SCNN1B) 并开发了一个高度准确的预测名录 (AUCtrain=0.991,AUCval=0.978).
- 发现枢纽基因与免疫细胞 (T细胞,B细胞) 之间存在显著的关联,这表明免疫透的作用.
结论:
- 确定了E2F3,CHIA和SCNN1B作为SS患者中CC的关键基因.
- 在这个队列中开发了一个有效的CC风险预测的经过验证的nomogram.
- 提出不平衡的免疫反应是一种共享的致病机制,为SS的CC提供新的诊断和治疗途径.
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