细胞内pH值将能量代谢与淋巴细胞死亡和增殖联系在一起
Wei-Ping Zeng1, Shuangshuang Yang2, Baohua Zhou2
1Therazwimm Corporation, 3128 Ferguson Road, Huntington, WV, 25705, USA. weipingzengny@gmail.com.
Scientific reports
|September 25, 2025
概括
细胞内pH值 (pHi) 影响淋巴细胞存活率和免疫反应的大小. 高pHi促进了淋巴细胞的生存,而低pHi诱导了亡,影响了免疫反应的控制.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 代谢调节 代谢调节 代谢调节
背景情况:
- 淋巴细胞细胞内pH值 (pHi) 在调节免疫反应大小中的作用尚不清楚.
- 能量代谢对pHi的影响以及随后的淋巴细胞增殖和死亡需要研究.
研究的目的:
- 确定能量代谢是否会影响淋巴细胞pHi,进而控制淋巴细胞死亡和增殖,从而调节免疫反应的大小.
- 为了研究pHi,线粒体膜潜力 (MMP) 和免疫反应期间的淋巴细胞命运之间的关系.
主要方法:
- 使用体外抗CD3激活和体内卵蛋白敏感化/挑战小鼠模型研究的淋巴细胞.
- 使用的代谢调节剂 (二乙酸盐,C75,CB-839,GSK2837808A) 调节能量代谢和pHi.
- 评估了淋巴细胞亡,扩散,pHi和MMPs.
主要成果:
- 低的pHi诱导了淋巴细胞增殖中的亡;高的pHi促进了生存.
- 代谢调节剂向酸盐和脂肪酸的流入,或抑制谷氨酸溶解/有氧糖溶解,所有降低了pHi.
- 淋巴细胞增殖与高MMPs在低pHi导致了亡,而高pHi与增加的淋巴细胞数量和积极的pHi-MMP关系相关挑战后.
结论:
- 谷氨醇溶解和有氧糖溶解对于维持有利的pHi对于淋巴细胞存活和免疫反应进展至关重要.
- 这些代谢途径抵消了pyruvate和脂肪酸代谢的酸性作用.
- pHi是淋巴细胞命运和免疫反应大小的关键调节者,受细胞能量代谢的影响.
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