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基于人类乳头瘤病毒的纳米颗粒载体的开发和表征,用于异种疫苗抗原

Yu Wang¹, Nils Bartelsen¹, Philipp Arnold²

¹Harald zur Hausen Institute of Virology, University Hospital Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, 91054, Erlangen, Germany.

Scientific reports

|September 25, 2025

概括

基于人类乳头瘤病毒 (HPV) 的纳米颗粒有效地呈现人类免疫缺陷病毒1型 (HIV-1) 包膜抗原,增强B细胞激活和抗体反应,用于新型疫苗开发.

科学领域:

疫苗学疫苗学疫苗学
纳米技术纳米技术
免疫学免疫学免疫学

背景情况:

在纳米颗粒上重复显示抗原可增强B细胞激活和幽默免疫力.
病毒样颗粒 (VLP) 技术,特别是人类乳头瘤病毒 (HPV) 的技术,已为疫苗开发奠定了基础.

研究的目的:

开发一种基于HPV的纳米粒子载体,呈现人体免疫缺陷病毒1型 (HIV-1) 包膜糖蛋白 (Env) 作为异质疫苗抗原.
评估这些新型L1-Env纳米粒子的免疫性和B细胞激活潜力.

主要方法:

纯化的HPV-16 L1囊蛋白被组装成VLP (L1-VLP).
使用无铜点击化学与DBCO终结链接器,HIV-1 Env剪切剂与L1-VLP表面结合,生成L1-Env纳米粒子.
在小鼠中进行了B细胞激活测试和免疫研究.

主要成果:

与未结合的Env相比,L1-Env纳米颗粒证明了对Env特定B细胞的更有效的激活.
用L1-Env纳米粒子免疫在没有辅助剂的小鼠中引起了Env和L1特异性抗体反应.
L1-Env纳米颗粒诱导的Env特异性抗体反应明显高于接受Envtrimers和未合的L1-VLP混合物的对照组.
观察到L1特异性抗体的减少,这表明受结合Env.的部分表皮层屏蔽.

结论:

基于HPV的纳米颗粒作为一个有效的平台来呈现异质抗原,如HIV-1 Env.
这种方法增强了B细胞激活和抗体产生,为开发针对性传播感染的新型组合疫苗提供了有前途的战略.

关键词:

在T助手纳米粒子.激活B细胞的激活过程没有铜的点击反应反应.DBCO DBCO 的意思是DBCO.环境管理员功能化的功能化.艾滋病毒疫苗接种者艾滋病毒疫苗接种者在HPV-16 L1囊体中.幽默性免疫反应是一种免疫反应.类似病毒的粒子 (VLP)

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