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高水平的生产Tauroursodeoxycholic酸从Taurochenodeoxycholic酸在工程 Bacillus subtilis通过全细胞生物转化

Lina Jin¹, Tengfei Niu¹, Rufeng Wang¹

¹The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicine, Shanghai Key Laboratory of Compound Chinese Medicines, State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Institute of Chinese Materia Medica, The MOE Key Laboratory for Standardization of Chinese Medicines, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, P.R. China.

World journal of microbiology & biotechnology

|September 25, 2025

概括

工程 Bacillus subtilis 将胆转化为tauroursodeoxycholic acid (TUDCA),这是一个熊胆汁成分. 这为生产有价值的药物化合物TUDCA提供了一种可持续的,具有成本效益的方法.

科学领域:

生物技术是生物技术.
合成生物学合成生物学
生物化学生物化学

背景情况:

熊胆粉是一种富含tauroursodeoxycholic acid (TUDCA) 的传统中医药.
高需求和道德问题需要替代品来承受胆汁.
塔乌罗斯氧胆酸 (TUDCA) 有潜在的治疗应用.

研究的目的:

为了设计Bacillus subtilis,有效地将陶氏氧化醇酸 (TCDCA) 生物转化为TUDCA.
开发一种具有成本效益和可扩展的方法,从易于获得的来源生产TUDCA.
创造一种可持续的替代品,用于医疗用途的熊胆.

主要方法:

在Bacillus subtilis中使用7α/7β-hydroxysteroid脱酶为TCDCA转化为TUDCA的两步酶途径进行了工程.
优化了核糖体结合点 (RBS) 和实施了葡萄糖1-脱酶 (GDH) 介导的NAD(P) H辅因子再生.
使用全细胞生物催化系统进行发酵和TUDCA生产.

主要成果:

获得了高产量的TUDCA (15.10 ± 0.36 g/L和14.33 ± 1.24 g/L) 与有利的TUDCA/TCDCA比率 (1.22 和 0.88).
中间T-7-KLCA无法检测,表明有效的通路功能.
从胆粉中证明了TUDCA的成功生物生产,使用工程B. subtilis.

结论:

工程B. subtilis菌株为工业TUDCA生产提供了一个可行的和可扩展的平台.
这家微生物细胞工厂为熊胆提供了一个可持续和道德的替代方案.
开发的生物转化过程具有成本效益,利用胆作为原料.

关键词:

这种细菌是 Bacillus subtilis.熊胆粉是什么意思熊胆粉是什么意思胆粉胆粉是什么意思在NAD(P) H辅因子再生过程中,核糖体结合部位是核糖体结合部位.塔乌尔苏二氧化醇酸是什么塔乌尔苏二氧化醇酸

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