菜大静脉相关病毒ORF3编码一个功能性的30K运动蛋白
Willem E W Schravesande1,2, Machiel V Cligge1, Raoul Frijters2
1Molecular Plant Pathology, Swammerdam Institute for Life Sciences (SILS), University of Amsterdam, Amsterdam, the Netherlands.
Molecular plant pathology
|September 25, 2025
概括
菜大静脉相关病毒ORF3作为运动蛋白,促进植物病毒通过局部化到plasmodesmata传播. 这一发现有助于理解病毒的运动和疾病的发展.
科学领域:
- 植物病毒学 植物病毒学
- 分子生物学分子生物学
- 结构生物学是结构生物学.
背景情况:
- 运动蛋白 (MPs) 对于植物病毒细胞间和系统性运动至关重要.
- 30K超级家族代表了最大的MP群体,其特点是尽管序列相似性较低,但仍保留了状结构.
- 大静脉相关病毒 (LBVaV) 与严重的大静脉疾病 (LBVD) 有关,并促进其他病毒的传播,但其蛋白质仍然没有表征.
研究的目的:
- 为了识别和表征大静脉相关病毒 (LBVaV) 的运动蛋白.
- 调查LBVaV ORF3在植物病毒运动中的作用及其在30K超级家族MP中的潜在分类.
主要方法:
- 使用AlphaFold2进行蛋白质结构预测,并使用FoldSeek进行基于结构的同质性搜索,以注释LBVaV开放阅读框架 (ORF).
- 进行了显微镜检查,以确定LBVaV ORF3.3的亚细胞局部.
- 在植物表达研究中进行,以评估ORF3促进运动受损病毒运动的能力.
主要成果:
- 所有LBVaV的ORF都被注释,ORF3被确定属于30K超级家族的国会议员.
- LBVaV ORF3与相关的MP具有较低的序列相同性 (5-11%),但具有特有的果卷折叠.
- 显微镜证实ORF3定位在plasmodesmata,其表达增强了两个不同的植物病毒的细胞间移动.
结论:
- 在AlphaFold2-FoldSeek策略成功地发现了一个新的MP (LBVaV ORF3) 尽管低序列同质.
- LBVaV ORF3 作为运动蛋白,局部化到等离子体,促进病毒传播.
- 这一发现有助于了解植物中的MP和病毒运动机制的多样性.
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