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类酸盐CtrCBL1/CtrCIPK6复合物CtrBBX32用于调节CtrSTP1介导的糖累积和耐寒性

Xiangming Shang^1,2, Zeqi Zhao^1,2, Wei Xiao^1,2

¹National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, 430070, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|September 26, 2025

概括

植物根通过通过CtrSTP1.1.积累糖来增强耐寒性. 一个涉及CtrCBL1/CtrCIPK6,CtrBBX32和CtrZAT10的新型调节模块在冷应力下控制这种糖运输.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
植物生理学植物生理学

背景情况:

植物耐寒性对生存至关重要,在根部积聚糖是关键的适应策略.
在寒冷压力下调节根糖平衡的精确分子机制尚未完全理解.

研究的目的:

在寒冷条件下识别和描述控制三叶根中糖累积的监管网络.
阐明特定蛋白质和转录因子在植物耐寒性中的作用.

主要方法:

在Citrus trifoliata中识别和表达冷诱导糖载体 (CtrSTP1) 的表达分析.
对转录因子与CtrSTP1促进体 (CtrBBX32和CtrZAT10) 的结合分析.
研究蛋白质-蛋白质相互作用和酸化事件 (CtrCBL1/CtrCIPK6复合体).
转基因方法来确认冷耐受性基因功能.

主要成果:

根部局部化的糖载体CtrSTP1通过促进糖的积累来积极调节耐寒性.
CtrBBX32作为抑制剂,CtrZAT10作为CtrSTP1表达的激活剂,CtrBBX32也抑制了CtrZAT10.
CtrCBL1/CtrCIPK6复合物化并降解CtrBBX32,缓解CtrZAT10和CtrSTP1.1上的抑制作用.
通过调节糖路径,CtrCIPK6积极影响寒冷耐受性.

结论:

一个新的调节模块 (CtrCBL1/CtrCIPK6-CtrBBX32) 通过CtrSTP1表达控制冷诱导的根糖积累.
该模块直接或间接调节CtrSTP1,影响糖运输和根在寒冷压力下的恒温.
这些发现为植物适应不利环境的机制提供了关键的见解.

关键词:

类植物三叶式植物寒冷的压力是冷的压力.蛋白质激酶酶是一种蛋白质激酶.糖的运输蛋白质是糖的运输蛋白质.转录规则转录规则转录规则

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