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氨基酸载体活性与放射性治疗在差异化甲状腺癌中的疗效之间的关系

Akihito Kotani¹, Yota Tatara², Riki Sakamoto¹

¹Department of Radiation Science, Hirosaki University Graduate School of Health Sciences, Hirosaki, Aomori 036‑8564, Japan.

Oncology reports

|September 26, 2025

概括

这项研究研究了与放射性 (I) 治疗的差异化甲状腺癌 (DTC) 患者的代谢变化. 研究人员确定了与良好的治疗反应相关的高分支链氨基酸 (BCAA),这表明潜在的新生物标志物独立于铁血球蛋白抗体.

科学领域:

内分泌学在内分泌学.
在瘤学瘤学.
代谢学代谢学代谢学

背景情况:

分化甲状腺癌 (DTC) 是最常见的内分泌恶性瘤,主要用全甲状腺切除术治疗.
手术后的监测依赖于血清红蛋白 (Tg) 和抗Tg抗体 (Tg/Ab) 水平,但Tg/Ab干扰影响了25%的DTC患者.
放射性 (¹³¹I) 治疗是残留疾病的标准,通常是在重组人甲状腺刺激激素 (rhTSH) 后进行的.

研究的目的:

探索DTC患者接受I疗法的代谢变化的分子机制.
确定治疗反应的潜在生物标志物,这些生物标志物独立于Tg/Ab干扰.
在DTC细胞模型中研究细胞对电离辐射的反应.

主要方法:

在RhTSH刺激后的I治疗之前和之后从DTC患者收集的血清.
进行了血清代谢分析,以确定代谢物水平的变化.
利用TPC-1细胞系研究细胞对电离辐射 (IR) 和氨基酸吸收的反应.
评估了细胞表面抗原和载体基因表达 (CD98,LAT1).

主要成果:

血清代谢分析显示,在具有良好的临床结果的患者中,分支链氨基酸 (BCAA) 水平 (氨酸,白氨酸,单氨酸) 升高.
在IR中存活的TPC-1细胞显示氨基酸吸收增加,与患者的发现相关.
被辐射的TPC-1细胞表现出更高的CD98细胞表面抗原表达,这表明它在BCAA吸收中发挥了作用.
在IR后没有观察到LAT1,LAT2或CD98hc的mRNA表达的显著变化.

结论:

升高的BCAA可能与DTC患者接受I治疗的良好治疗反应有关.
照射DTC细胞中增加的BCAA吸收似乎是一种暂时的,细胞表面介导的反应,涉及LAT1/CD98hc.
CD98表达可能在调解DTC细胞对电离辐射的反应中对BCAA吸收起作用.

关键词:

L型氨基酸载体1 氨基酸载体1 有分支链的氨基酸.有差异化的甲状腺癌.放射性同位素治疗是一种放射性同位素治疗.

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