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相关概念视频

Conserved Binding Sites01:49

Conserved Binding Sites

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Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
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EDTA titrations are usually carried out in highly basic conditions, where the fully deprotonated form of EDTA, Y4−, actively complexes with the free metal ions in the solution. Several metal ions precipitate as hydrous oxide (hydroxides, oxides, or oxyhydroxides) under these conditions, lowering the concentration of free metal ions in the solution. For this reason, auxiliary complexing agents or ligands such as ammonia, tartrate, citrate, or triethanolamine are used in EDTA titrations to...
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RNA-seq03:21

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Tail-anchored, or TA, proteins are estimated to make up to 3-5% of membrane proteins found in the eukaryotic cell. Such proteins have a single transmembrane domain located approximately 30 amino acid residues upstream from the C-terminal end. As a result, the signal recognition particle (SRP) cannot guide a TA protein to the ER membrane for cotranslational insertion. Hence, they are integrated into the ER membrane post-translationally using their C-terminal end as the anchor. TA proteins...
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EMICSS:对EMDB条目进行增值注释.

Amudha Kumari Duraisamy1, Neli Fonseca1, Gerard J Kleywegt1

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概括
此摘要是机器生成的。

电子显微镜数据库 (EMDB) 现在通过EMDB集成与复杂,结构和序列 (EMICSS) 进行了增强的交叉引用. 该系统自动将3DEM数据与重要的生物和结构资源联系起来.

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科学领域:

  • 结构生物学 结构生物学
  • 生物信息学是一种生物信息学.
  • 数据科学数据科学数据科学

背景情况:

  • 电子显微镜数据库 (EMDB) 是3D电子显微镜 (3DEM) 数据的主要存储库.
  • 目前EMDB的局限性包括缺乏全面的注释和与外部生物,功能和结构数据库的连接.
  • 这些局限性源于可选数据存储和维护外部引用的挑战.

研究的目的:

  • 开发一个自动化系统,以加强EMDB数据集成和交叉引用.
  • 通过将3DEM数据与相关资源联系起来,提高3DEM数据的可发现性和实用性.
  • 为应对存款人负担的挑战,并保持最新的外部参考资料.

主要方法:

  • 开发了EMDB集成与复杂,结构和序列 (EMICSS),一个独立的系统.
  • 实现了与20多个外部资源的自动交叉引用 (例如,UniProt,AlphaFold DB,PubMed,复杂门户,基因本体学).
  • 通过API,使EMICSS注释在多种格式和程序上可访问.

主要成果:

  • EMICSS成功地自动化并更新EMDB条目的交叉引用.
  • 由EMICSS生成的注释被集成到EMDB网站中,增强了入口页面,统计和搜索功能.
  • 该系统提供访问链接资源和程序化数据检索.

结论:

  • 通过提供全面的注释和与外部生物数据库无集成,EMICSS显著增强了EMDB.
  • 自动化系统提高了结构生物学和相关领域的研究人员的数据可访问性和实用性.
  • EMICSS是一个基于Python的开源工具,促进协作开发和广泛采用.