合成自营酵母可以通过综合途径和工艺设计,从CO2中产生高水平的伊塔康酸
Özge Ata1,2, Lisa Lutz1,2, Michael Baumschabl1,2
1BOKU University, Vienna, Institute of Microbiology and Microbial Biotechnology, Department of Biotechnology and Food Science 1190 Vienna Austria oezge.ata@boku.ac.at.
概括
在这项研究中,Komagataella phaffii被设计成直接从二氧化碳 (CO2) 中产生伊塔康酸. 这种合成自营酵母获得了显著的标位,证明了使用二氧化碳作为原料的可持续生物工艺.
科学领域:
- 生物技术和合成生物学
- 代谢工程是代谢工程.
- 可持续化学 可持续化学
背景情况:
- 单一碳 (C1) 基板对于生物化学品生产日益重要.
- 二氧化碳 (CO2) 是一种可持续的替代原料,可以减轻温室气体排放.
- 开发用于直接转化二氧化碳的微生物平台对于可持续的过程至关重要.
研究的目的:
- 设计一种合成的自性Komagataella phaffii (Pichia pastoris) 菌株,从二氧化碳直接生产伊塔康酸.
- 优化代谢流量和工艺设计,以提高伊塔康酸标位.
- 为了证明K. phaffii作为利用CO2的微生物平台的潜力.
主要方法:
- 对于自营生长和伊塔康酸生产的Komagataella phaffii的代谢工程.
- 卡尔文-本森-巴什姆 (CBB) 循环与伊塔康酸生物合成途径的整合.
- 生物反应器培养和过程优化,以最大限度地提高产品产量.
主要成果:
- 在生物反应器培养中达到大约12克L-1的伊塔克酸的最终标位.
- 使用工程K. phaffii.证明成功地将二氧化碳直接转化为伊塔康酸.
- 确定了提高生产效率的关键代谢平衡和工艺设计策略.
结论:
- 科马加泰拉 (Komagataella phaffii) 可以被设计成一个强大的微生物平台,用于利用二氧化碳的可持续生物工艺.
- 平衡CBB循环流量和伊塔康酸代谢对于高效的基于CO2的生产至关重要.
- 这项工作支持向基于C1的可持续化学制造转变,使用废弃CO2.
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