基于毛细体的pH响应的纳米酶性SERS传感器用于诊断Helicobacter pylori
Yangfan Zhou1, Caili Bi1, Tengfei Pang2,1
1Institute of Translational Medicine, Medical College, Yangzhou University, Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases Yangzhou 225001 P. R. China.
RSC advances
|September 26, 2025
概括
一种新型的pH响应型纳米酶传感器准确检测唾液中的尿酶,这是Helicobacter pylori感染的关键指标. 这种基于SERS的快速方法在临床应用中显示出高的诊断效果.
科学领域:
- 纳米技术纳米技术
- 生物医学工程 生物医学工程
- 分析化学 分析化学
背景情况:
- 在唾液中精确检测尿酶对于诊断Helicobacter pylori (H. pylori) 感染至关重要.
- 目前的诊断方法可能缺乏广泛查所需的灵敏度或速度.
研究的目的:
- 开发一个pH响应的纳米酶表面增强的拉曼散射 (SERS) 传感器,用于间接检测尿酶.
- 为了评估传感器在唾液样本中诊断H. pylori感染的性能.
主要方法:
- 在毛细血管内壁上制造纳米酶金芯外纳米棒 (Au@Pd NRs).
- 利用尿酸酶催化尿素水解的纳米酶活性的pH依赖抑制来调节SERS信号.
- 通过氧化TMB (ox-TMB) SERS信号的变化间接检测尿酶度.
主要成果:
- 传感器在15分钟内达到6.09U L-1的低检测极限 (LOD).
- 唾液样本的结果与标准尿素酶测试工具显著相关.
- 接收器操作特征 (ROC) 分析得出曲线下的面积 (AUC) 为0.959,表明诊断潜力很高.
结论:
- 开发的纳米酶SERS传感器提供了一种快速,灵敏和准确的方法来检测唾液中的尿酶.
- 该传感器显示出对H. pylori感染的有希望的临床诊断疗效.
- 这项技术有可能改善H. pylori的查和诊断.
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