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使用定量全细胞结构分析对胰岛素分泌颗粒的生物物理亚群进行分类

Kevin Chang¹, Aneesh Deshmukh¹, Riva Verma²

¹Department of Chemistry, Bridge Institute, USC Michelson Center for Convergent Bioscience, University of Southern California, Lo Angeles, CA 90089, USA.

bioRxiv : the preprint server for biology

|September 26, 2025

概括

研究人员开发了新的方法来分类胰腺β细胞中的胰岛素分泌颗粒 (ISG) 亚群. 这种方法揭示了外部信号如何动态地改变ISG分布,有助于开发有针对性的疗法.

科学领域:

细胞生物学细胞生物学
生物物理学的生物物理.
内分泌学在内分泌学.

背景情况:

胰腺β细胞在胰岛素分泌颗粒 (ISG) 中产生和储存胰岛素.
通过生物物理重塑,ISG成熟,创造了具有不同分子和空间特征的多样化子群.
定义这些ISG亚群的系统方法目前还不发达.

研究的目的:

开发和应用系统方法来分类胰岛素分泌颗粒 (ISG) 亚群.
研究ISG亚群体在对分泌刺激的反应中发生的动态重塑.
整合多种成像技术,以全面了解ISG异质性.

主要方法:

软X射线断层扫描 (SXT) 用于量化测量整个β细胞中的ISG生化密度.
无监督集群根据分子密度,大小和空间定位对ISG亚群进行分类.
使用体积电子显微镜 (vEM) 来表征初级β细胞,将发现与SXT数据相结合.

主要成果:

通过使用SXT和无监督集群,建立了ISG子群的分类框架.
外源性胰岛素分泌刺激诱导ISG亚群向成熟和可释放状态的动态转变.
整合SXT和vEM为ISG异质性提供了洞察力,这些洞察力仅仅通过这两种方法无法获得.

结论:

开发的方法提供了一个强大的框架来定义和分析ISG亚群.
伊斯格亚群的动态重塑对外部分泌信号有反应.
这种方法为开发治疗策略提供了基础,以增强有益的ISG亚群,以改善胰岛素分泌.

关键词:

胰岛素分泌颗粒胰岛素分泌颗粒胰腺β细胞是什么软X射线断层扫描 (X-ray Tomography) 是一种软X射线断层扫描.没有监督的集群.全细胞成像全细胞成像密集核心颗粒的颗粒.

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