在巨细胞内复制的Candida glabrata经历了氨基酸剥夺,DNA损伤和染色体不稳定
Ariel A Aptekmann1, Nathaly Cabrera1, Melody C Hayman2,3
1Center for Discovery and Innovation, Hackensack Meridian Health, Nutley, NJ, USA.
bioRxiv : the preprint server for biology
|September 26, 2025
概括
虫虫通过激活氨基酸生物合成和DNA修复途径,在巨吞后存活下来. 巨细胞通道会导致DNA损伤和染色体不稳定,而RME1突变会增强体能.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 遗传学 遗传学 是一个
背景情况:
- 巨细胞是关键的先天性免疫细胞,可以吞细菌.
- 机会性酵母 Candida glabrata 能够在巨体内生存和复制,尽管这种敌对的环境.
- 对C. glabrata对巨吞的反应机制及其对基因组稳定性的影响尚不清楚.
研究的目的:
- 为了研究C. glabrata对巨吞的反应.
- 为了确定巨细胞环境对C. glabrata基因组稳定性的影响.
- 识别导致C. glabrata在巨细胞内生存和复制的遗传因素.
主要方法:
- 进行比较的转录学以确定C. glabrata的应激反应.
- 对DNA双链断裂修复和DNA损伤的测试 (彗星测试).
- 脉冲场凝电泳和长读DNA测序以分析染色体变化.
- 突变分析用于识别巨细胞通过过程中的遗传变化.
主要成果:
- 氨基酸饥饿和DNA损伤模仿了C. glabrata对巨吞的反应.
- 巨细胞内生存需要GCN4调节器和DNA修复.
- 在巨细胞的酵母中观察到高的DNA断裂和频繁的染色体变化.
- 在RME1中发生的移突变增强了C. glabrata在巨细胞中的适应性.
结论:
- C. glabrata 在巨细胞中经历了氨基酸剥夺和DNA损伤.
- 巨细胞通道导致基因组不稳定,包括复杂的染色体变化.
- RME1基因参与半分裂调节,在C. glabrata的适应性和巨细胞中的持久性中发挥作用.
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