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通过Gli3R介导的刺信号的抑制会改变斑马鱼的胚胎转录组

Anna J Moyer¹, Summer B Thyme¹

¹Department of Biochemistry and Molecular Biotechnology, The University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.

bioRxiv : the preprint server for biology

|September 26, 2025

概括

刺信号调节斑马鱼的发育. 抑制Gli3抑制剂减少了神经元和离子细胞的基因,这表明这种途径在透恒温中发挥了新的作用.

科学领域:

发育生物学发展生物学
遗传学是一个遗传学.

背景情况:

刺信号对于脊椎动物胚胎发育至关重要.
它在斑马鱼胚胎中的破坏会影响神经管细胞类型.

研究的目的:

研究刺信号传导的作用,特别是Gli3抑制剂活性,在斑马鱼胚胎发生过程中的作用.
识别通过这种途径调节的新型细胞类型和发育过程.

主要方法:

在斑马鱼胚胎中Gli3抑制剂的无处不在的过度表达.
在受精后30小时的胚胎中进行大量RNA测序 (RNA-seq).
基因组丰富分析使用Daniocell atlas标记基因.

主要成果:

减少与侧面地板板,运动神经元,科尔默-阿格杜尔细胞,多巴胺基神经元,缓慢肌肉细胞和前垂体相关的基因表达.
在富含H+-ATPase和富含Na+-K+-ATPase的离子细胞中对基因的下调,对透性恒温至关重要.
减少离子细胞特异性载体基因和转录因子foxi3a的表达.

结论:

Gli3抑制剂活性对于各种神经元和肌肉细胞类型的发展至关重要.
通过Gli活动,刺信号传递在斑马鱼胚胎的离子细胞发育和透恒温中发挥了以前未知的作用.

关键词:

在Fox3的世界里.这是一个很棒的节目,Gli Gli Gli.在Gli3R中,Gli3R是指Gli3R.富含H+-ATP 酶的蛋白质是什么?在RNA-seqqq.胚胎发育过程中的胚胎.刺是一种刺,刺是一种刺.离子恒温是离子恒温.离子细胞离子细胞斑马鱼是一种斑马鱼.

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