在体外和体内衍生的巨细胞占据了不同的表型状态
Stéphane Chevrier1, Vito R T Zanotelli1,2, Daniel Schulz1
1Department of Quantitative Biomedicine, University of Zürich, Winterthurerstrasse 190, 8057 Zürich, Switzerland.
bioRxiv : the preprint server for biology
|September 26, 2025
概括
单细胞衍生巨细胞 (MDMs) 模型显示出明显的表面标记物,但与瘤相关巨细胞 (TAMs) in vivo不同. 这项研究澄清了MDM概况,并突出了研究巨细胞生物学现有模型的局限性.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 单细胞衍生巨细胞 (MDMs) 对于研究人体巨细胞生物学在体外 (in vitro) 中至关重要.
- 标准化的两极化条件旨在模仿in vivo巨细胞激活状态.
- 缺乏跨共识种群的MDM表面标志物表达的系统分析.
研究的目的:
- 使用质量细胞计,全面描述MDM表面标记物配置文件.
- 为了研究MDM表面标记物表达的时间动态.
- 为了在体外比较MDM表型与患者衍生的瘤相关巨细胞 (TAMs) 在体内比较.
主要方法:
- 质量细胞计 (CyTOF) 用于表面标记物的高维单细胞分析.
- 人体单细胞的标准化体外分化和两极化转化为MDMs.
- 患者体内MDM和体外TAM之间的表型比较.
主要成果:
- 为核心in vitro分化MDM种群阐明了详细的表面表达特征.
- 系统地分析了MDM表面标记物表达的时间变化.
- 在体外MDM和体内TAM之间观察到细胞表面特征的显著差异,包括标记物表达水平和组合,尽管它们具有共同的标记物.
结论:
- 这项研究提供了MDM表面表型的高维澄清.
- 这些发现揭示了当前体外模型在完全回顾体内TAM表型的复杂性方面的局限性.
- 这项研究为改进巨细胞模型和理解活体巨细胞生物学提供了途径.
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