直接RNA-seq提供了酵母Hrp1蛋白对RNA聚合酶II转录的抗终结作用的证据
Emma C Goguen1, Kylie A Zawisza1, David A Brow1
1Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, WI 53706.
bioRxiv : the preprint server for biology
|September 26, 2025
概括
Hrp1是一种RNA结合蛋白,对基因表达调节至关重要. 在Hrp1突变影响RNA聚合酶II终结和mRNA处理,影响基因表达和RNA处理. 这项研究揭示了Hrp1.
科学领域:
- * 分子和细胞生物学
- *RNA生物学 *RNA生物学
- * 酵母遗传学公司
背景情况:
- *Hrp1/Nab4是酵母中必不可少的核RNA结合蛋白.
- *最初被确定为裂变和多化因子 (CF1B),它还调节了非编码转录的终止.
- * 它在蛋白质编码基因的3'-end形成和反终结中的确切作用仍然不完全理解.
研究的目的:
- * 为了研究特定的Hrp1突变 (hrp1-7和hrp1-M191T) 的全转录组影响.
- *阐明Hrp1在RNA聚合酶II抗终结和mRNA处理中的功能.
- * 了解Hrp1突变如何影响终结,衰减和多基化位点选择.
主要方法:
- *利用纳米孔直接RNA测序来分析具有hrp1-7和hrp1-M191T突变的酵母菌株的转录组.
- * 进行了体外结合试验,以评估Hrp1变异对 (UA) n重复的亲和力.
- * 分析了mRNA多基化位点选择的变化,并确定了新的Hrp1-依赖的调节元素.
主要成果:
- *Hrp1-7和Hrp1-M191T蛋白质对 (UA) n重复的结合亲和力降低.
- * 确定了新的依赖Hrp1的小核细胞RNA终结器和mRNA减弱剂.
- *观察到mRNA多基化位点选择的广泛变化,对于hrp1-7和hrp1-M191T有不同的模式.
结论:
- *Hrp1突变破坏了RNA聚合酶II抗终结和mRNA3'-end处理.
- * 减少Hrp1对 (UA) n重复的亲和力以及与延长复合体的改变相互作用,有助于观察到的表型.
- *Hrp1通过终止,减弱和多基化位点选择在调节基因表达方面发挥着重要作用.
相关概念视频
Eukaryotic RNA Polymerases
26.8K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
All three eukaryotic RNAPs require specific transcription factors, of which the...
26.8K
Eukaryotic RNA Polymerases
9.1K
9.1K
Transcription Initiation
20.3K
Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...
The promoters and enhancers and their accessory proteins allow tight regulation of...
20.3K
Transcription Attenuation in Prokaryotes
18.1K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
18.1K
Bacterial RNA Polymerase
32.5K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
32.5K
RNA Polymerase II Accessory Proteins
10.8K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
10.8K


