相关实验视频

Updated: Jan 16, 2026

Determination of the Optimal Chromosomal Locations for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
11:12

Determination of the Optimal Chromosomal Locations for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Published on: September 11, 2017

7.9K

在人类细胞中的基因安全港口中插入基因大小的DNA,使用位点定向的转基因酶

James E Short, Lisa Sharek, Joshua F Meckler

    bioRxiv : the preprint server for biology
    |September 26, 2025
    PubMed
    概括

    研究人员开发了通过有针对性的定和条件转换 (INTACT) 进行插入,以精确地插入基因. 这种新的方法有效地将大型DNA序列集成到人类基因组中,而无需双链断裂,从而推进基因疗法工具.

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    Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing08:19

    Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

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    Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing09:03

    Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

    Published on: May 10, 2020

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    Conservative Site-specific Recombination and Phase Variation02:53

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    Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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    Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
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