替代拼接是一种驱动力,它调整了皮肤植物Trichophyton rubrum中的代谢适应和毒性特征
Marcos E Ramos Lopes1, João Neves-da-Rocha1, Pablo R Sanches1
1Department of Genetics, Ribeirão Preto Medical School, University of Sao Paulo, USP, Ribeirão Preto, SP, Brazil.
Frontiers in cellular and infection microbiology
|September 26, 2025
概括
替代拼接 (AS) 调节了Trichophyton rubrum中的真菌适应和病变发生. 这个过程会影响毒性和新陈代谢,突出显示AS.
科学领域:
- 菌类学 菌类学是指菌类学.
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 替代拼接 (AS) 是致病真菌的关键调节机制,但其在适应和毒性方面的确切作用在很大程度上仍未被探索.
- 了解AS对于破译宿主环境中的真菌生存策略至关重要.
研究的目的:
- 研究替代拼接 (AS) 在致病性真菌Trichophyton rubrum生理学和毒性的作用.
- 在类似感染的条件下识别和描述AS事件.
主要方法:
- 在 keratin (体外感染模型) 中种植的 Trichophyton rubrum 的 RNA 测序数据上进行了全转录组拼接分析.
- 扩展了对使用人类指甲和HaCaT角质细胞的ex vivo感染模型的分析.
主要成果:
- 替代拼接 (AS) 在类似感染的条件下调节大约12.6%的Trichophyton rubrum基因组.
- AS影响各种细胞过程,包括新陈代谢,细胞信号传递,蛋白质处理,转录和翻译.
- 控制毒性和新陈代谢的关键转录因子 (PacC,Ap1) 是由结合体机器控制的.
结论:
- 由AS产生的mRNA异型在宿主相互作用期间对Trichophyton rubrum的适应有显著的贡献.
- 转录因子基因的替代拼接在真菌病变发生过程中起着关键作用.
- 转录和拼接机械之间的相互作用优化了感染期间的真菌代谢和毒性特征.
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