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Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.

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Updated: Jan 16, 2026

Gentle Isolation of Nuclei from the Brain Tissue of Adult African Turquoise Killifish, a Naturally Short-Lived Model for Aging Research

Gentle Isolation of Nuclei from the Brain Tissue of Adult African Turquoise Killifish, a Naturally Short-Lived Model for Aging Research

Published on: August 9, 2022

在非洲绿鱼大脑中的基因表达检测方法

Emily Whisenant¹, Arne C Lekven¹

¹Department of Biology and Biochemistry, University of Houston, Houston, Texas, USA.

Developmental dynamics : an official publication of the American Association of Anatomists

|September 26, 2025

概括

研究人员优化了成本效益高的方法来可视化非洲青色鱼 (Nothobranchius furzeri) 的基因和蛋白质表达. 这些新协议增强了这种新兴模型生物体中的基因表达研究.

科学领域:

神经科学是一个神经科学.
遗传学是一个遗传学.
发育生物学发展生物学

背景情况:

非洲青色鱼 (Nothobranchius furzeri) 是基因表达研究的宝贵模型.
对于这种物种的转录和蛋白质检测,存在有限的成本效益,高分辨率的工具.
分析不透明,复杂的大脑组织需要先进的成像技术,但现有的方法耗时且设备密集.

研究的目的:

开发和优化可访问的,高分辨率的基因和蛋白质可视化方法在N. furzeri.
为了解决目前用于脑组织分析的转录和蛋白质检测技术的局限性.
扩大N.furzeri研究的方法工具包.

主要方法:

针对mRNA检测,优化了与低温切割兼容的现场杂交 (ISH) 协议.
调整了EZ-clear方法用于全脑蛋白质可视化.
使用Gfap和Dat标记器验证的协议,评估信号噪声比和表达模式.

主要成果:

在厚度和薄度的部分实现了对色度ISH的高信号噪声比,证实了预期的大脑区域表达.
成功适应EZ-clear用于N.furzeri脑组织清除,与免疫染色相容.
在转基因记者系中观察到潜在的Gfap上调和保存的内源光.

关键词:

EZ 清除清除清除一个成熟的成年人.光是一种光.免疫组织化学免疫组织化学在现场混合化.组织清除组织清除转录视觉化转录可视化

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Multiplex Detection of Gene Expression in the Intact Drosophila Brain Using Expansion-Assisted Iterative Fluorescence In Situ Hybridization

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Published on: May 2, 2025

Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue

Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue

Published on: April 27, 2012

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Last Updated: Jan 16, 2026

Gentle Isolation of Nuclei from the Brain Tissue of Adult African Turquoise Killifish, a Naturally Short-Lived Model for Aging Research

Gentle Isolation of Nuclei from the Brain Tissue of Adult African Turquoise Killifish, a Naturally Short-Lived Model for Aging Research

Published on: August 9, 2022

Multiplex Detection of Gene Expression in the Intact Drosophila Brain Using Expansion-Assisted Iterative Fluorescence In Situ Hybridization

Multiplex Detection of Gene Expression in the Intact Drosophila Brain Using Expansion-Assisted Iterative Fluorescence In Situ Hybridization

Published on: May 2, 2025

Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue

Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue

Published on: April 27, 2012

结论:

在N. furzeri. 中开发了用于基因和蛋白质可视化的经济有效和可访问的协议.
优化的ISH和EZ-clear方法增强了对这种模型生物体基因表达的研究.
这些协议扩大了N. furzeri. 的可用研究工具.