猪组织中长非编码RNA的综合性表征:卵细胞发育期间的表达模式和功能洞察
Yao Jiang1,2,3, Yipeng Li1, Qingpeng Shen1
1State Key Laboratory of Swine and Poultry Breeding Industry, Guangdong Laboratory of Lingnan Modern Agriculture, National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Cells
|September 26, 2025
概括
这项研究对猪的长非编码RNAs (lncRNAs) 进行了表征,确定了数千种组织特异性和家用 lncRNAs. 卵细胞lncRNAs的一个子集在物种中保存,可能调节关键的生物过程.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 动物科学动物科学
背景情况:
- 长非编码RNAs (lncRNAs) 是哺乳动物生物过程中的关键调节分子.
- 缺乏在各种猪组织中 lncRNA 表达的系统性表征.
研究的目的:
- 在多个猪组织中全面分析lncRNA表达模式.
- 在猪卵细胞中识别保存和差异表达的lncRNAs.
- 研究 lncRNAs 在猪生殖生物学中的功能影响.
主要方法:
- 来自14个组织的607个猪组织RNA-seq数据集的分析.
- 对63个猪卵细胞单细胞RNA-seq数据集的检查.
- 与人类和小鼠卵细胞RNA-seq数据集进行比较分析.
主要成果:
- 鉴定了52,798个猪 lncRNAs,在卵细胞中具有突出的组织特异性表达.
- 2,169个家庭和14469个组织特异性 lncRNAs 的分类.
- 在人类和小鼠中发现了保存的卵细胞lncRNAs,与催化活性和昼夜调节有关.
- 在卵细胞发育过程中鉴定出44种不同表达的lncRNAs,它们参与神经发生和蛋白质修饰.
结论:
- 这项研究提供了猪 lncRNA 和它们的表达特征的基础目录.
- 保存的卵细胞 lncRNAs 表明在物种间的基本生物过程中发挥了作用.
- 不同表达的lncRNA突出显示了猪卵细胞发育和生殖生物学中的潜在调节功能.
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