在猪中,Circ-06958通过miR-31-5p/AK4轴调节细胞增殖,从而影响肉质
Xiaohan Zhang1, Rongru Zhu1, Xiaoxu Wu1
1College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
Cells
|September 26, 2025
概括
循环RNAs (CircRNAs) 调节基因表达. 这项研究确定Circ-06958是促进肌肉细胞增殖的关键因素,通过一种新的调节途径影响猪肉质量.
科学领域:
- 遗传学和分子生物学
- 动物科学动物科学
- 生物化学 生物化学
背景情况:
- 循环RNAs (CircRNAs) 是通过竞争性内源RNA (ceRNA) 机制的基因表达的关键调节者.
- 环RNAs在猪肉质量发展中的作用在很大程度上仍未被探索.
- 了解这些机制对于改善畜牧生产和质量至关重要.
研究的目的:
- 描述不同肉质和肌纤维成分的猪肌肉中的CircRNAs.
- 为了研究特定的CircRNA,Circ-06958在肌肉细胞增殖及其调节途径中的功能.
- 阐明CircRNA对肉质形成的贡献背后的分子机制.
主要方法:
- RNA测序 (RNA-seq) 用于识别猪长胸和半肌中差异表达的CircRNAs.
- 关于Circ-06958的实验性表征,包括在猪骨肌卫星细胞 (PMSC) 和C2C12神经质细胞中的定位和功能测定.
- 使用分子生物学技术构建和验证Circ-06958/miR-31-5p/亚基酶4 (AK4) 调节轴.
主要成果:
- 鉴定了1126个差异表达的CircRNA,Circ-06958在两种研究的肌肉中都高度表达.
- 由长链乙-CoA合成酶1 (ACSL1) 基因衍生出的Circ-06958被证明可以通过促进细胞周期进展来增强肌肉细胞的增殖.
- 建立了一个新的调节轴,Circ-06958/miR-31-5p/AK4,证明Circ-06958通过miR-31-5p抑制和AK4上调促进增殖.
结论:
- Circ-06958在促进肌肉细胞增殖方面发挥着重要作用,这是肉质的关键决定因素.
- 已识别的miR-31-5p/AK4通路为肌肉发育中的CircRNA介导基因调节提供了新的见解.
- 这些发现有助于更深入地了解猪肉质量的分子基础,并为遗传改进提供潜在的目标.
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