使用挥发性有机化合物进行鼻癌的非侵入性检测
Chuan Hao Gui1, Zhunan Jia2, Alex Chengyao Tham1
1Tan Tock Seng Hospital, Singapore 308433, Singapore.
Metabolites
|September 26, 2025
概括
质子转移反应质谱学 (PTR-MS) 呼吸分析在鼻癌 (NPC) 患者中检测到明显的挥发性有机化合物 (VOC) 特性,显示出对非侵入性诊断的希望. 需要进一步的研究以获得临床确认.
科学领域:
- 在瘤学瘤学.
- 分析化学 分析化学
- 生物标志物 生物标志物
背景情况:
- 鼻癌 (NPC) 是亚洲普遍存在的头癌,通常在晚期被诊断出来,导致患者的治疗结果不佳.
- 早期检测策略对于提高NPC的存活率至关重要.
- 像呼吸分析这样的非侵入性方法为早期NPC诊断提供了潜力.
研究的目的:
- 为了研究挥发性有机化合物 (VOC) 样本在呼气中的实用性,以区分鼻癌 (NPC) 患者与健康个体.
- 评估通过质子转移反应质谱法 (PTR-MS) 检测到的VOC特征的诊断潜力.
主要方法:
- 从50名NPC患者和40名健康对照人群收集了呼吸样本.
- 使用质子转移反应飞行时间质谱法 (PTR-TOF-MS) 分析出口的VOC.
- 统计分析包括单变量比较,多变量回归,主要成分分析 (PCA) 和随机森林分类,调整混因素.
主要成果:
- 七个不同的VOC特征 (m/z值) 在NPC患者和对照人群之间存在显著差异,其中m089和m175是关键的区分因素.
- 主要成分分析 (PCA) 表明,在正常化后,组之间的分离得到改善.
- 随机森林模型在NPC检测方面实现了中度的分类准确性,在对共变量进行调整后表现强.
结论:
- 质子转移反应质谱学 (PTR-MS) 呼吸分析可以在鼻癌 (NPC) 中识别疾病特定的VOC特征.
- 这种非侵入性呼吸分析技术显示出作为NPC的潜在诊断工具的希望.
- 需要进行更大规模的验证研究和对VOC化合物的最终鉴定,以确定临床效用.
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