转录因子MAHMG,高流动性组蛋白质,与化模式转移和Metarhizium acridum中的应激耐受性有关
Rongrong Qiu1,2,3, Jinyuan Zhou1,2,3, Tingting Cao1,2,3
1Genetic Engineering Research Center, School of Life Sciences, Chongqing University, Chongqing 401331, China.
Journal of fungi (Basel, Switzerland)
|September 26, 2025
概括
在Metarhizium acridum中的高流动性群蛋白 (HMG) 对真菌发育和抗压力至关重要. 删除MAHMG会显著减少化,并降低应激耐受性,突出其在生物控制潜力中的作用.
科学领域:
- 菌类生物学 菌类生物学
- 分子菌学分子菌学
- 生物控制剂 生物控制剂
背景情况:
- 结合和应激耐受性是昆虫病原菌的关键特征,影响生产和环境生存.
- 高流动性群蛋白 (HMG) 在虫病原菌中的作用在很大程度上是未知的,尽管它们在性发育中的作用已知.
研究的目的:
- 为了研究MaHMG基因在Metarhizium acridum中的功能.
- 为了确定MAHMG对空调和应力耐受性的影响.
主要方法:
- 在Metarhizium acridum中对MahMG的基因淘汰.
- 对结合开始,产量和模式的分析.
- 评估状芽和对UV-B,热冲击和白的耐受性.
- RNA测序 (RNA-seq) 用于识别差异表达基因 (DEG).
主要成果:
- 删除MaHMG延迟了结合并减少了58%的结合性产量.
- 突变者显示出改变的结合模式和降低的发芽率.
- ΔMaHMG突变体对UV-B和热冲击的耐受性降低,对CFW的敏感性增加.
- 通过RNA-seq检测,发现了88种DEGs,这些DEG与细胞壁生物发生,细胞循环和结合有关.
结论:
- 在Metarhizium acridum中,MAHMG作为一种关键的阳性调节器,对结合和应激耐受性起作用.
- 在真菌生物学中,MAHMG发挥着根本性的作用.
- MaHMG是提高生物控制剂性能的潜在目标.
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