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分泌蛋白VdCUE调节了Verticillium dahliae的毒性,而不会干扰BAX诱导的细胞死亡

Haonan Yu¹, Haiyuan Li², Xiaochen Zhang¹

¹State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Yangling 712100, China.

Journal of fungi (Basel, Switzerland)

|September 26, 2025

概括

这项研究确定VdCUE是Verticillium wilt.的关键毒性因子. 在Verticillium dahliae中淘汰VdCUE显著降低了棉花植物的疾病严重程度.

科学领域:

植物病理学植物病理学
分子微生物学分子微生物学
生物化学生物化学

背景情况:

由Verticillium dahliae引起的Verticillium枯,对农作物和树木构成重大全球威胁.
了解V. dahliae病原性的分子机制对于开发有效的控制策略至关重要.

研究的目的:

描述V. dahliae中CUE (乌比奎结合与内质网膜降解的合) - 包含域蛋白VdCUE的功能.
调查VdCUE在V. dahliae生长,毒性和宿主相互作用中的作用.

主要方法:

∆VdCUE-Knockout突变体的生成和表型评估.
在Nicotiana benthamiana中对VdCUE分泌和细胞死亡诱导试验的功能分析.
使用酵母二混合查和基于AI的结构建模来识别宿主目标.

主要成果:

∆VdCUE突变体表现出减少了黑色化微硬质细胞的形成,但没有显著的生长缺陷.
∆VdCUE突变病毒感染导致棉花植物的疾病指数和脱叶率明显降低.
VdCUE是一种分泌的蛋白质,与宿主位相互作用,包括tRNA特异的2-thiouridylase,peptidyl-prolyl cis-trans异构酶和40S核糖体蛋白.

结论:

在V. dahliae中,VDCUE是关键的毒性因子,对于完全的致病性至关重要.
已识别的宿主目标可能会调解VdCUE-依赖的毒性调节.
这项研究增强了我们对Verticillium枯病原体的理解.

关键词:

这种植物名为Verticillium dahliae.微小的克勒奥提亚是什么意思被编程的细胞死亡.分泌的蛋白质是分泌的蛋白质毒性毒性是一种毒性.

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