补充Candida介质增强免疫反应并调节SARS-CoV-2疫苗接种小鼠的肠道微生物群
Renan E A Piraine1,2, Neida L Conrad1, Vitória S Gonçalves1
1Microbiology Laboratory, Technological Development Center, Federal University of Pelotas, Pelotas 96010-610, RS, Brazil.
Journal of fungi (Basel, Switzerland)
|September 26, 2025
概括
非Saccharomyces酵母Candida中间体显示出益生菌潜力,增强免疫反应和针对SARS-CoV-2接种疫苗的小鼠的抗体产生. 这种酵母可以作为一种新的益生菌和疫苗辅助剂.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- 非Saccharomyces酵母因其益生菌能力而闻名,可能超过Saccharomyces boulardii的益生菌能力.
- 介质菌 (Candida intermedia) 是一种非白菌酵母,它以前已经证明了益生菌的潜力,并且不是典型的病原体.
- 在SARS-CoV-2疫苗接种模型中评估C.intermedia的免疫调节作用,进一步证明了其益生菌活性.
研究的目的:
- 为了评估Candida intermedia ORQ001在与非活化SARS-CoV-2接种疫苗的小鼠中的免疫调节作用.
- 研究C.intermedia对免疫基因表达,抗体产生和肠道微生物组合的影响.
主要方法:
- 用C.intermedia刺激小鼠巨细胞,并使用定量PCR (qPCR) 分析mRNA表达.
- 在接受非活化SARS-CoV-2疫苗接种之前,小鼠接受了C.中介补充剂.
- 通过ELISA测量了抗体水平 (IgG,sIgA),使用下一代测序分析了便微生物组.
主要成果:
- C. intermedia显著改变了巨细胞基因表达,增加了Il4和Il13,同时减少了Stat3.
- 得到补充的小鼠的脊髓细胞显示Tnf,Ifng,Il4,Bcl6和Stat3.3的转录增加.
- 补充剂的小鼠表现出更高的抗SARS-CoV-2IgG和sIgA水平,并在他们的肠道微生物群中丰富了细菌和菌物种的种群.
结论:
- 在小鼠模型中,Candida中间体补充剂有效调节了免疫基因表达,并增强了幽默免疫反应.
- 观察到对胃肠道微生物群的影响表明,在提高疫苗免疫性方面发挥了协同作用.
- 这些发现支持C. intermedia作为一种有前途的新型益生菌,具有与疫苗辅助性相关的免疫调节性质.
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