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Size-Exclusion Chromatography01:08

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In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
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Children at play often make suspensions such as mixtures of mud and water, flour and water, or a suspension of solid pigments in water known as tempera paint. These suspensions are heterogeneous mixtures composed of relatively large particles visible to the naked eye or seen with a magnifying glass. They are cloudy, and the suspended particles settle out after mixing. The suspended particles in a suspension settle out after some time of mixing. The separation of particles from a suspension is...
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(宏观) 分子拥挤效应超出了体积排除.

Boris Y Zaslavsky1, Vladimir N Uversky2

  • 1Cleveland Diagnostics, Cleveland, OH, USA.

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概括
此摘要是机器生成的。

大分子拥挤显著影响蛋白质的行为,超出了简单的排除体积效应. 本综述探讨了在拥挤的生物环境中扩散,粘度和溶剂性质变化等额外因素.

关键词:
水性双相系统是水性双相系统.排除了体积效应的可能性.巨分子拥挤是什么意思分区分区分区分区分区软互动是一种柔软的互动.溶剂属性 溶剂的属性粘度 粘度 粘度 粘度 粘度

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科学领域:

  • 生物化学 生物化学
  • 生物物理学的生物物理.
  • 分子生物学分子生物学

背景情况:

  • 大分子拥挤影响蛋白质折叠,结合,酶活性和聚合.
  • 传统观点认为,拥挤效应主要归因于被排除的体积相互作用.
  • 为了更深入地理解,需要考虑除了被排除的数量之外的其他因素.

研究的目的:

  • 在拥挤的环境中审查和突出影响蛋白质行为的关键因素.
  • 扩大对大分子拥挤的理解,超出排除的体积效应.
  • 强调crowders在溶剂特性和直接相互作用方面的作用.

主要方法:

  • 对宏分子拥挤研究的文献综述.
  • 在拥挤的溶液中分析影响蛋白质动态和相互作用的因素.
  • 综合当前关于未被排除的体积效应的知识.

主要成果:

  • 拥挤的环境引入扰乱的扩散和改变的溶液粘度.
  • 软相互作用和crowders和蛋白质之间的直接物理接触是显著的.
  • 群众大量修改溶剂特性,影响蛋白质的行为.

结论:

  • 在拥挤的溶液中蛋白质的行为是多个因素的复杂相互作用.
  • 排除的体积不足以完全解释对蛋白质的拥挤效应.
  • 考虑扰乱的扩散,粘度,相互作用和溶剂效应对于全面的理解至关重要.