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Updated: Jun 13, 2026

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector
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用流通微通道和较低交替电压的基因电转移产生了来自人类淋巴细胞细胞系的诱导多能细胞

Miho Ishii-Teshima1,2, Koki Maeda1, Kazuki Hanauchi3

  • 1Department of Mechanical Engineering, Toyohashi University of Technology, Toyohashi, Japan.

PloS one
|September 26, 2025
PubMed
概括

一个新的微通道流通电穿孔器使得从淋巴细胞细胞系 (LCLs) 产生诱导多能干细胞 (iPSCs) 的经济有效的产生成为可能. 这项技术降低了成本,并允许对各种LCL基因型进行并行处理,推动了iPSC研究.

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Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
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Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
Forced Transdifferentiation01:28

Forced Transdifferentiation

Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial transdifferentiation occurs...