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核糖体蛋白L27有助于Eimeria tenella中的毒性和马杜拉米辛耐药性

Yu Yu¹, Guo Huilin¹, Liujia Li²

¹Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology of Ministry of Agriculture, Minhang, Shanghai, 200241, China.

International journal for parasitology. Drugs and drug resistance

|September 26, 2025

概括

核糖体蛋白L27 (EtRPL27) 与家禽寄生虫Eimeria tenella的耐药性有关. 过度表达EtRPL27增强了寄生虫的毒性和maduramicin耐药性,这表明它是一个治疗点.

科学领域:

* 禽类健康与寄生虫学
* 分子生物学和药物耐药性

背景情况:

*由Eimeria spp.引起的菌病显著影响全球家禽产量.
*耐药的Eimeria菌株降低了抗菌药治疗的有效性.
* 核糖体蛋白L27 (EtRPL27) 此前已被确定为药物耐药Eimeria tenella中的差异表达.

研究的目的:

* 调查EtRPL27在Eimeria tenella毒性和maduramicin耐药性中的作用.
* 探索EtRPL27作为潜在的诊断标记物和菌病的治疗点.

主要方法:

*逆转录定量PCR (RT-qPCR) 用于特定阶段的表达分析.
* 免疫光显微镜用于EtRPL27定位.
*使用抗EtRPL27抗体和转基因EtRPL27过度表达的功能性试验.
* 对的入侵效率,致病性和药物敏感性的评估.

主要成果:

*EtRPL27的表达是特定阶段的,在杂虫和第二代杂虫中达到顶峰.
* EtRPL27在细胞质中发现,部分在E. tenella的表面.
* 抗马杜拉米辛菌株表现出显著更高的EtRPL27转录水平.
*抗EtRPL27抗体抑制杂虫虫的入侵;EtRPL27过度表达增强了入侵和致病性.
*EtRPL27的过度表达赋予了对maduramicin的中等至高耐药性.

结论:

* EtRPL27是Eimeria tenella毒性和maduramicin耐药性的一个关键因素.
*EtRPL27作为药物耐药性的潜在分子标志物.
* 针对EtRPL27是一个有前途的策略,用于对抗耐药性菌病.

关键词:

药物耐药性药物耐药性药物耐药性埃梅里亚天尼拉 (Eimeria tenella) 是一种植物.马杜拉米西尼是什么意思马杜拉米西尼是什么意思核糖体蛋白质L27 是一种蛋白质.病毒性病毒性

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