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Filippo Silveri1, Flavio Della Pelle1, Carmine Merola1

  • 1Department of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Campus "Aurelio Saliceti" Via R. Balzarini 1, 64100, Teramo, Italy.

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这项研究引入了可持续的藻纸传感器,用于对斑马鱼胚胎及其环境中的双A (BPA) 实时监测. 这些新型传感器为在体内评估BPA生物度提供了快速,环保的解决方案.

关键词:
副产品 副产品 副产品在生物体内进行的研究.实验室制造的传感器纳米-生物炭的使用.纸质设备是一种基于纸的设备.斑马鱼胚胎 斑马鱼的胚胎

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科学领域:

  • 环境科学 环境科学
  • 分析化学 分析化学
  • 材料科学 材料科学 材料科学

背景情况:

  • 在体内模型中评估污染物水平需要智能实时监测解决方案.
  • 当前的分析方法往往依赖于场外的,耗时的仪器技术.
  • 需要快速的现场策略来监测生物系统中的污染物.

研究的目的:

  • 开发可持续的藻纸传感器,用于确定斑马鱼胚胎 (Z-EBs) 中的双甲 (BPA) 生物度.
  • 在体内研究期间,使Z-EBs在培养基中的BPA水平能够实时监测.
  • 提供一种可靠的方法来评估Z-EBs中的BPA生物积累.

主要方法:

  • 制造藻类纸传感器,使用模板印刷和从海藻废物中提取的生物炭.
  • 通过以水为基础的液相脱皮来制备生物炭,避免使用有机溶剂.
  • 在斑马鱼胚胎培养基和Z-EB中通过矩阵校准对BPA的电化学传感.

主要成果:

  • 藻类传感器在96小时内准确地跟踪了Z-EB培养基中的BPA变化 (相对误差为 -14/+12%).
  • 在Z-EB中实现了生物累积BPA的58nM/13μg L-1检测极限.
  • 已证明可重复结果 (RSD ≤8.8%) 和定量恢复 (94-118%),证实了传感器的可靠性.
  • 传感器有效地区分了与Z-EBs发育延迟相关的可能致命的BPA水平.

结论:

  • 开发了一种可持续的,基于循环经济的藻类纸传感器,用于BPA检测.
  • 在体内研究中展示了第一个便携式电化学装置,用于在体内测定BPA.
  • 藻纸传感器可以实时监测和评估BPA的最终暴露生物积累.