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通过细胞膜透性修饰和多酶细分的有效生产D-塔加

Dandan Tang¹, Li Yang², Rui Long²

¹College of Food Science and Light Industry, Nanjing Tech University, Nanjing, 211816, China; School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing, 211816, China.

International journal of biological macromolecules

|September 26, 2025

概括

经过工程设计的大肠杆菌通过改善细胞膜和组织酶,有效地产生罕见的糖D-塔加. 这种方法提高了低热量甜味剂的生产,实现了4.35倍提高D-塔加产量.

科学领域:

生物技术是生物技术.
代谢工程是代谢工程.
合成生物学合成生物学

背景情况:

D-塔加是一种低热量甜味剂,在功能性食品中具有潜在的应用.
微生物生产D-塔加面临着由于基质运输和酶活性低效的挑战.
改善D-塔加的生物合成需要增强细胞过程的策略.

研究的目的:

为了增强大肠杆菌中D-塔加的生物合成.
为了克服乳糖吸收和辅助因子失衡的限制.
通过空间组织来提高酶催化效率.

主要方法:

通过删除布朗脂蛋白 (lpp) 基因和过度表达乳糖浸透酶 (lacY) 来改造大肠杆菌,以改善乳糖的运输.
整合了与膜结合的跨酶基因 (pntAB) 来解决辅因子失衡.
使用RGG-RGG基因介导的液体-液体相分离来进行酶分离 (SgXR和RlGDH).

主要成果:

减少了三分之一的发酵时间,保持了产量.
通过辅因子平衡,增加了1.20倍的D-塔加标位.
达到了10.01g/L的D-塔加位,4.35倍的改善,理论收益率为95.2%,生产率为0.209g/L/h.

结论:

通过相分离将细胞膜工程与酶细分结合起来,对于稀有糖的生产是有效的.
这种方法显著提高了微生物细胞工厂中的D-塔加生产.
工程菌株BLPBT04展示了功能成分的工业规模生物合成的强大策略.

关键词:

细胞膜通透性的细胞膜通透性.D - 塔加托糖 D - 塔加托糖酶细分化酶的细分化乳糖是一种乳糖.

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