来自Coix种子-乳酸细菌发酵的同质多糖的结构特征和抗衰老活动
Yating Cheng1, Shanshan He2, Zhihui Wei2
1State Key Laboratory of Bioactive Molecules and Druggability Assessment, College of Life Science and Technology & College of Pharmacy, Jinan University, Guangzhou, 510632, China; Biopharmaceutical R&D Center of Jinan University, Guangzhou, China; Guangdong Engineering Research Center of Chinese Medicine & Disease Susceptibility/Guangzhou Key Laboratory of Traditional Chinese Medicine &Disease Susceptibility/Guangdong-Hong Kong-Macao Universities Joint Laboratory for the Internationalization of Traditional Chinese Medicine/International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Chinese Ministry of Education (MOE)/Guangdong Engineering Research Center of Traditional Chinese Medicine & Health Products/Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou, 510632, China.
纯化Coix种子多糖 (CP1) 通过延长寿命和增强抗压能力,显示出显著的抗衰老作用. 它激活了p-AMPK/RICTOR/CREB3通路,为衰老提供了一个潜在的治疗候选者.
科学领域:
- 生物化学 生化学
- 老年学是指老年学的学科.
- 药理学 药理学 是一个学科.
背景情况:
- 科伊种子是一种传统的中医药,因其抗衰老潜力而闻名.
- 目前尚不清楚Coix种子多糖的抗衰老特性背后的特定生物机制.
研究的目的:
- 系统地研究纯化Coix种子多糖的抗衰老作用 (CP1).
- 阐明CP1抗衰老作用的潜在分子机制.
主要方法:
- 使用甘氨酸键和分子量分析对CP1的结构性表征.
- 在体外抗氧化活性评估使用DPPH,ABTS和基清除试验.
- 在Caenorhabditis elegans和斑马鱼模型中的体内抗衰老研究.
- 在人体皮肤纤维细胞的体外研究,以评估DNA损伤减少.
- 机制性研究涉及途径分析 (p-AMPK/RICTOR/CREB3) 和蛋白相互作用 (PARP-1).
主要成果:
- CP1是一种葡萄糖多糖,平均分子量为8.855kDa.
- CP1对DPPH,ABTS和基激素表现出强大的清理活动.
- CP1显著延长了C. elegans的寿命,并提高了氧化应激下的生存率.
- 在斑马鱼中,CP1降低了与衰老相关的β-galactosidase活性,并减轻了人类皮肤纤维细胞中的DNA损伤.
- 通过与PARP-1的相互作用,CP1激活了p-AMPK/RICTOR/CREB3通路.
结论:
- 这项研究提供了CP1的第一个结构特征,并在多个模型系统中证明了其广泛的抗衰老作用.
- CP1的机制涉及到p-AMPK/RICTOR/CREB3通路的激活.
- CP1代表了开发新型抗衰老疗法的有希望的候选分子.
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