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乳糖辅助的β-galactosidase的固定与增强的转糖酶化性能
Chaoyue Wu1, Guangzhen Wang2, Hanwen Yang3
1College of Food Science and Engineering, Ocean University of China, 266003, Qingdao, China.
International journal of biological macromolecules
|September 26, 2025
概括
使用一种新的乳糖辅助方法将β-galactosidase固定起来,可以显著提高其稳定性和转甘油化活性. 这种改进的酶促进了乳糖的产生,为乳制品功能化提供了一个有前途的策略.
科学领域:
- 生物催化和酶工程 生物催化和酶工程
- 食品科学与技术 食品科学与技术
- 生物技术是生物技术.
背景情况:
- 固定酶在稳定性和可重复使用性方面具有优势,可用于工业应用.
- 增强β-银酸酶的转糖化活性是功能性食品成分生产的关键.
- 开发强大的固定化策略对于高效的生物催化剂设计至关重要.
研究的目的:
- 为了将Kluyveromyces lactis中的β-galactosidase固定在Duolite A568.8上.
- 优化固定条件,增强酶活性和稳定性.
- 为了研究乳糖生物合成的转糖化活性.
主要方法:
- 通过静电吸附和甲交叉连接来固定酶.
- 优化固定参数 (pH,酶度,时间).
- 介绍了一种新的乳糖辅助交叉链接策略.
主要成果:
- 优化的固定产生了高的酶负载能力 (约27毫克/克树脂).
- 乳糖辅助交联 (ACP-β-galactosidase) 导致相对活性达到226%,并提高了操作稳定性.
- ACP-β-galactosidase的乳糖产量达到60.45g/L,明显高于自由酶的产量.
结论:
- 一个强大的乳糖辅助固定化策略被开发为高性能β-galactosidase生物催化剂.
- 固定有效调节β-银酸酶的功能,改善乳糖生物合成.
- 这种方法为提高乳制品的功能性质提供了一种新方法.
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