相关实验视频

Updated: Jan 16, 2026

Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis

Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis

Published on: February 10, 2015

向LOXL1表达的肝 Stellate 细胞抑制纤维化和鼻状血管生成通过LOXL1/RUNX1/VEGFA轴在肝纤维化进展期间

Xuzhen Yan¹, Qi Han², Yiwen Wang²

¹Experimental and Translational Research Center, Beijing Friendship Hospital, Capital Medical University, Beijing, P.R. China; Beijing Clinical Medicine Institute, Beijing, P.R. China; State Key Lab of Digestive Health, Beijing, P.R. China; National Clinical Research Center of Digestive Diseases, Beijing, P.R. China.

Cellular and molecular gastroenterology and hepatology

|September 26, 2025

概括

肝星细胞 (HSC) 中的酸氧化酶类1 (LOXL1) 通过促进HSC激活和内皮细胞变化,驱动肝纤维化. 准LOXL1/RUNX1/VEGF轴可以治疗肝纤维化.

科学领域:

肝病学肝病学是一种肝病学.
细胞生物学细胞生物学
生物化学生物化学

背景情况:

肝星细胞 (HSC) 是通过过度的细胞外基质 (ECM) 生产导致肝纤维化的关键驱动因素.
肝细胞和肝内皮细胞 (LSEC) 之间的交叉声在纤维化中至关重要,但机制尚不清楚.
类似酸氧化酶1 (LOXL1) 是一种参与ECM交叉链接的酶,在HSC-LSEC相互作用中具有潜在的作用.

研究的目的:

为了研究LOXL1在肝纤维化期间HSC-LSEC交叉中的作用.
阐明LOXL1影响肝纤维化进展的分子机制.

主要方法:

采用了HSC和LSEC的共同培养系统.
产生了特定于HSC的Loxl1淘汰赛小鼠,并接受了食或化学纤维化模型.
组织学,电子显微镜,免疫染色,qPCR和RNA测序被用于分析.

主要成果:

在培养过程中,HSC中的LOXL1调节了LSEC中的ECM.
在HSC中LOXL1的丧失减少了HSC激活,LSEC毛细化和纤维化.
RNA测序确定了RUNX家族转录因子1 (RUNX1) 作为LOXL1对HSC激活和血管生成的影响的调解者.

结论:

由HSC衍生的LOXL1通过通过RUNX1/VEGF轴增强HSC激活和LSEC毛细化来促进肝纤维化.
准LOXL1/RUNX1/VEGF通路为肝纤维化提供了潜在的治疗策略.

关键词:

细胞外矩阵细胞外矩阵肝脏星状细胞是什么意思肝脏纤维化肝脏纤维化肝脏侧鼻内皮细胞内皮细胞阴侧状毛细化阴侧状毛细化

更多相关视频

Novel In Vivo Micro-Computed Tomography Imaging Techniques for Assessing the Progression of Non-Alcoholic Fatty Liver Disease

Novel In Vivo Micro-Computed Tomography Imaging Techniques for Assessing the Progression of Non-Alcoholic Fatty Liver Disease

Published on: March 24, 2023

Development of an Ethanol-induced Fibrotic Liver Model in Zebrafish to Study Progenitor Cell-mediated Hepatocyte Regeneration

Development of an Ethanol-induced Fibrotic Liver Model in Zebrafish to Study Progenitor Cell-mediated Hepatocyte Regeneration

Published on: May 13, 2016

相关概念视频

Liver Regeneration

Liver Regeneration

The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...