在Grapholita molesta中,V-ATPase B调解了Bt Cry1Ac结合和毒性
Dandan Pan1, Jiayang Feng1, Jiacheng Ye1
1Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Insect biochemistry and molecular biology
|September 26, 2025
概括
研究了Bacillus thuringiensis (Bt) Cry1Ac蛋白对Grapholita molesta害虫的毒性.研究人员还研究了Bacillus thuringiensis (Bt) Cry1Ac蛋白对Grapholita molesta害虫的毒性. 研究人员确定GmolV-ATPase B是围性膜上的关键结合蛋白,增强了Cry1AcAc.
科学领域:
- 昆虫毒理学 昆虫毒理学
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- 格拉福利塔 (Grapholita molesta) 是一个重要的农业害虫.
- 甲状腺菌 (Bt) Cry1Ac蛋白提供了一个替代的杀虫剂.
- 了解Cry1Ac与昆虫中肠蛋白的相互作用对于生物杀虫剂的开发至关重要.
研究的目的:
- 为了识别与Cry1Ac相互作用的蛋白质在G. molesta.的周围膜 (PM) 上.
- 阐明已识别的蛋白质在Cry1Ac中介毒性中的作用.
- 探索向这些蛋白质以提高生物杀虫剂有效性的潜力.
主要方法:
- 用于蛋白质识别的联结斑,SDS-PAGE和LC-MS/MS.
- 用RT-qPCR进行基因表达分析.
- 用于细胞毒性测定和RNA干扰 (RNAi) 的Sf9细胞培养.
- 在体内生物测试使用G. molesta幼虫.
主要成果:
- 鉴定出GmolV-ATPase B是一种Cry1Ac结合蛋白在G. molesta周围膜上.
- 摄入Cry1Ac减少了GmolV-ATPase B的表达;通过竞争试验证实了相互作用.
- 过度表达GmolV-ATPase B增加了Cry1Ac细胞毒性,而RNAi降低了幼虫死亡率.
- 结合的GmolV-ATPase B和Cry1Ac显著增强了对G. molesta的Cry1Ac毒性.
结论:
- GmolV-ATPase B 是G. molesta.中的Cry1Ac的一个功能性受体.
- 这种相互作用对Cry1Ac杀虫活性至关重要.
- 准GmolV-ATPase B是一种有前途的战略,用于开发针对G. molesta.的新型生物杀虫剂.
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