开发一种可溶解的微针贴片,用于通过皮肤输送SARS-CoV-2mRNA疫苗,增强稳定性和免疫性
Zequn Tang1, Tong Su1, Ting Jiang2
1Department of Pharmaceutics, School of Pharmaceutical Sciences Fudan University & Key Laboratory of Smart Drug Delivery, Ministry of Education, Shanghai 201203, China.
概括
溶解微针贴片提供了一个稳定的,患者友好的mRNA疫苗输送方法. 与传统注射相比,这种新贴片提高了疫苗的稳定性和免疫性.
科学领域:
- 生物技术是生物技术.
- 疫苗学 疫苗学 疫苗学
- 材料科学 材料科学 材料科学
背景情况:
- 使用微针 (MNs) 的透皮输送系统是对mRNA疫苗肌内注射 (IM) 的有希望的替代方案.
- 多种类型的护理提供了改善患者服药性,皮肤向性输送,疫苗稳定性和减少LNP肝脏积累.
研究的目的:
- 开发一种可溶解的微针贴片 (DMP) 用于SARS-CoV-2 mRNA疫苗 (RQ3013) 的输送.
- 为了优化制造过程,提高mRNA-LNP封装和体内性能.
主要方法:
- 使用聚乙烯醇 (PVA) 和聚乙烯 (PVP) 基质通过一步真空微型成型工艺制造DMP.
- 评估mRNA-LNP封装效率,体内成像,物理化学特性,细胞吸收,抗原表达,稳定性和免疫性.
- 与两步过程和IM交付进行比较.
主要成果:
- 与两步过程 (72.34%) 相比,单步DMP实现了显著更高的mRNA-LNP封装效率 (95.54%) .
- 在体内成像显示,单步DMP的累积光强度比双步DMP高2.2倍,比IM高2.6倍.
- DMP保留了mRNA-LNP的完整性和功能,在较低剂量下诱导了与IM相似的IgG反应,并且在室温下在30天内表现出良好的稳定性,没有毒性或刺激.
结论:
- 开发的DMP是一种有效,稳定和患者友好的mRNA疫苗输送策略.
- 这种方法增强了免疫性,改善了储存稳定性,并简化了疫苗的使用.
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