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封装增强可切换蛋白质释放工程化益生菌乳杆菌

Marc Blanch-Asensio¹, Varun Sai Tadimarri¹, Roberto Martinez²

¹INM - Leibniz Institute for New Materials, Saarland University, Campus D2 2, 66123 Saarbrücken, Germany; Saarland University, 66123 Saarbrücken, Germany.

Journal of controlled release : official journal of the Controlled Release Society

|September 26, 2025

概括

研究人员用基因开关设计了益生菌Lactiplantibacillus plantarum,用于控制药物释放. 将这些工程细菌封装在PEARL (蛋白质排泄酸与复合乳酸菌) 中,显著降低了泄漏率,从而实现了精确的按需治疗.

科学领域:

合成生物学合成生物学
微生物治疗学微生物治疗学
生物材料工程生物材料工程

背景情况:

乳酸菌等益生菌细菌缺乏用于控制蛋白质生产的先进遗传工具.
大肠杆菌虽然在遗传上具有多样性,但并不适合所有治疗应用.
在非模型益生菌中开发按需释放药物系统对于先进的治疗方法至关重要.

研究的目的:

为了设计Lactiplantibacillus plantarum具有响应性遗传开关,以实现精确的控制.
研究材料封装对工程益生菌的性能的影响.
为使用乳杆菌的刺激响应微生物治疗平台奠定基础.

主要方法:

在L. plantarum中具有强大的构成性促进体 (P_tec) 的综合性酸盐或酸盐反应性遗传电路.
在各种生长条件下评估开关性能,包括营养限制和温度变化.
在核心外的酸盐珠 (PEARLs) 中固定化工程L. plantarum以评估泄漏抑制和控制.

主要成果:

在L. plantarum中开发了两种基因开关,具有微粒感应范围.
在快速生长的作物中观察并描述了与酸化相关的泄漏性.
使用PEARLs封装显著抑制泄漏和日间可逆控制.
在共同封装和小型化的PEARL中展示了直角开关的稳定性能.

结论:

扩大了益生菌L. plantarum的基因工程能力.
强调了将遗传电路设计与生物材料封装相结合的协同效益.
促进了乳酸菌的发展,作为刺激反应和精确控制微生物治疗的平台.

关键词:

阿尔金酸盐是一种酸盐.工程化生物材料工程化生物材料遗传开关是一个遗传开关.益生菌的乳酸菌菌.蛋白质分泌蛋白质分泌

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