微量奥洛素对由矿/硫驱动的自性脱过程的影响:性能,微生物群落进化和元基因组分析
Wenyu Yang1, Xin Xin1, Xishuang Cao1
1School of Resources and Environment, Chengdu University of Information Technology, Chengdu 610225, China.
Journal of environmental sciences (China)
|September 26, 2025
概括
抗生素ofloxacin (OFL) 对地下水脱产生负面影响,降低了酸盐去除效率,并抑制了关键酶. 虽然低水平的OFL增加了微生物多样性,但较高度会损害微生物多样性,并抑制了脱基因,而不会显著增加抗生素耐药性基因.
科学领域:
- 环境微生物学 环境微生物学
- 水处理技术水处理技术
- 环境化学环境化学
背景情况:
- 在地下水中经常检测到像牛素 (OFL) 这样的抗生素.
- 使用金/硫 (FeS2/S0) 的自转型脱系统对于酸盐去除至关重要.
- 了解OFL对这些系统的影响对于水质管理至关重要.
研究的目的:
- 为了研究ofloxacin (OFL) 对化性能在化/硫驱动的自系统的影响.
- 确定OFL对微生物活性和基因表达的度依赖影响.
- 评估在OFL暴露下抗生素耐药性基因 (ARG) 的扩散.
主要方法:
- 暴露于自性脱系统的不同度的奥洛素 (200 ng/L - 100 μg/L).
- 测量酸盐去除效率,电子运输活性 (ETSA) 和关键酶活性 (NAR,NIR).
- 分析微生物多样性,功能性基因表达 (脱和硫氧化) 和抗生素耐药性基因 (ARG).
主要成果:
- 奥夫洛克萨显著抑制了酸盐去除效率,这种效率取决于度.
- 增加的OFL水平降低了ETSA,缩酶 (NAR) 和缩酶 (NIR) 的活动.
- 虽然低度的OFL增强了微生物多样性和硫氧化基因,但高度会降低它们;脱基因表达被普遍抑制.
- 细胞外蛋白 (PN) 含量增加,表明应激反应,并且没有观察到ARG的显著增殖.
结论:
- 奥夫洛克萨对自性脱产生抑制作用,影响微生物群落和关键功能基因.
- 该系统通过PN生产表现出一些弹性,但高OFL度不利于脱效率.
- 特定的细菌群体,如Thiobacillus,Anaerolineae,Anaerolineales和Nitrospirae可能会在这个系统中存在现有的ARG.
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